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F2518

Human Fibronectin

Name: Human Fibronectin
Brand: SIGMA

from human foreskin fibroblasts, powder, suitable for cell culture

Sinónimos:

Fibronectin

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UNSPSC Code:

12352202

NACRES:

NA.75

EC Number:

289-149-2

MDL number:

MFCD00131062

Biological source:

human foreskin fibroblasts

Form:

lyophilized powder

Technique(s):

cell culture | mammalian: suitable

Mol wt:

~550 kDa

Servicio técnico

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Propiedades

Nombre del producto

Fibronectina, lyophilized powder, suitable for cell culture

biological source

human foreskin fibroblasts

Quality Level

200

product line

BioReagent

form

lyophilized powder

mol wt

~550 kDa

packaging

pkg of 0.5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm²

impurities

Human Source, none detected (HCV), Human Source, none detected (HIV), Human Source, none detected (HTLV), Human Source, none detected (HbSAg), Microbial Contamination, passes test

solubility

H₂O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI accession no.

NM_212482.4

UniProt accession no.

P02751

binding specificity

Peptide Source: Collagen

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... FN1(2335)

Descripción

General description

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.

Application

Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

Features and Benefits

Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
The starting material is tested and found negative for HIV and HBsAg.
The product is soluble in sterile water, yielding a clear, colorless solution.
It can be stored at -20°C for at least three years.

Physical form

Lyophilized in CAPS buffered saline

Preparation Note

Store reconstituted solution in working aliquots at -20°C or lower.

dialyzed against chloroform

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Disclaimer

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

0000420443

0000218931

0000129869

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Contenido relacionado

Data Sheet - F2518

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