H0887
HEPES solution
1M, liquid, pH 7.0-7.6, suitable for cell culture, BioXtra, BioReagent
Sinónimos:
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
About This Item
Número CAS:
UNSPSC Code:
12161704
PubChem Substance ID:
NACRES:
NA.75
MDL number:
Nombre del producto
HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
SMILES string
OCCN1CCN(CCS(=O)(O)=O)CC1
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
sterility
sterile-filtered
product line
BioReagent
BioXtra
assay
0.9—1.1 M
form
liquid
storage condition
(Tightly closed. Dry)
dry at room temperature
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
colorless
pH
7.0-7.6
useful pH range
6.8-8.2
7.5
density
1.1 g/cm3
suitability
suitable for component for culture media
suitable for electrophoresis
application(s)
cell analysis
life science and biopharma
sample preparation
storage temp.
2-8°C
Quality Level
¿Está buscando productos similares? Visita Guía de comparación de productos
Application
HEPES has been used as a component of:
- Hank’s balanced salt solution, Dulbecco’s modified eagle’s medium and no-glucose DMEM, which are used for the preparation of tissue slices
- Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity
- cell culture media to provide buffer capacity to maintain the pH of the cell line
- buffer in decellularization of porcine nucleus pulposus
Disclaimer
Concentrations of HEPES over 40mM may be toxic to some cell types.
Features and Benefits
- High purity product for biochemical and biological research
- Sterile filtered and cell culture tested
- Ready Solution reduces the need
- Useful for the pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
- Ready available solution reduce the need for preparation time
General description
HEPES Buffer Solution, a sterile-filtered 1M solution of 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, serves as a vital biological buffer in cell biology, biochemical, and biological research. This zwitterionic buffer enjoys widespread adoption due to its effective pH range of 6.8 to 8.2, making it suitable for a diverse array of research applications. HEPES excels as a buffering agent in cell culture media, offering several advantages over alternatives like Tris and phosphate. Notably, HEPES exhibits lower cell toxicity, ensuring optimal cell health and viability during experiments.
Furthermore, it surpasses bicarbonate buffers (NaHCO3) in its ability to maintain physiological pH despite fluctuations in carbon dioxide concentration arising from cellular respiration, a key factor in maintaining cellular homeostasis. Beyond its exceptional performance in cell culture, HEPES may also find application in various biological and biochemical studies. It may also be valuable in techniques like immunoprecipitation, cell lysis, and live cell imaging, providing a stable and controlled environment for experimental procedures. The combination of its versatility, exceptional pH buffering capacity, and minimal interaction with other molecules positions HEPES as an indispensable tool across diverse research fields.
Furthermore, it surpasses bicarbonate buffers (NaHCO3) in its ability to maintain physiological pH despite fluctuations in carbon dioxide concentration arising from cellular respiration, a key factor in maintaining cellular homeostasis. Beyond its exceptional performance in cell culture, HEPES may also find application in various biological and biochemical studies. It may also be valuable in techniques like immunoprecipitation, cell lysis, and live cell imaging, providing a stable and controlled environment for experimental procedures. The combination of its versatility, exceptional pH buffering capacity, and minimal interaction with other molecules positions HEPES as an indispensable tool across diverse research fields.
It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Preparation Note
Prepared from HEPES free acid and cell culture grade water.
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Kaixi Tang et al.
PLoS computational biology, 14(7), e1006303-e1006303 (2018-07-06)
Focal adhesions are protein complexes that anchor cells to the extracellular matrix. During migration, the growth and disassembly of these structures are spatiotemporally regulated, with new adhesions forming at the leading edge of the cell and mature adhesions disassembling at
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland Nt
Acta Biomaterialia, 8, 925- 936 (2012)
The role of the endocrine system in feeding-induced tissue-specific circadian entrainment.
Sato M, et al.
Cell Reports, 8(2), 393-401 (2014)
Zuzana Keckesova et al.
Nature, 543(7647), 681-686 (2017-03-23)
Post-mitotic, differentiated cells exhibit a variety of characteristics that contrast with those of actively growing neoplastic cells, such as the expression of cell-cycle inhibitors and differentiation factors. We hypothesized that the gene expression profiles of these differentiated cells could reveal
Chiao-Chen Chen et al.
Analytical chemistry, 85(7), 3621-3628 (2013-02-21)
Elucidation of epithelial transport across transcellular or paracellular pathways promises to advance the present understanding of ion transport and enables regulation of cell junctions critical to the cell and molecular biology of the epithelium. Here, we demonstrate a new instrumental
Contenido relacionado
Product Information Sheet
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico