H1387
Hanks′ Balanced Salts
Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture
Sinónimos:
HBSS
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Hanks′ Balanced Salts, Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
Quality Level
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Application
Hanks′ Balanced Salts has been used:
- for diluting digested tissues during isolation of pancreatic blood vessels
- for washing mouse intestinal mucosal cells before lysis and sonication
- as an immersion solution to test the in vitro bioactivity of poly(ε-caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone
General description
Balanced salt solution (BSS) serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It also provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. BSS when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism. In addition, it also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).
Other Notes
The pH is adjusted after addition of sodium bicarbonate. Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
Preparation Note
Formulated to contain 9.7 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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