H3662

HEPES sodium salt solution

Name: HEPES sodium salt solution
Brand: SIGMA

BioReagent, 1M, suitable for cell culture

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About This Item

Fórmula empírica (notación de Hill):

C₈H₁₇N₂NaO₄S

Número CAS:

75277-39-3

Peso molecular:

260.29

NACRES:

NA.25

PubChem Substance ID:

24895590

UNSPSC Code:

12161700

MDL number:

MFCD00036463

Nombre del producto

HEPES sodium salt solution, BioReagent, 1M, suitable for cell culture

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

sterility

sterile-filtered (0.2 μm filtered)

product line

BioReagent

form

solution

concentration

0.90-1.10 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested
DNase, RNase. Protease, Nickase, free
endotoxin, tested
<5 ppm Heavy metals (as Pb)

cation traces

Fe: <5 ppm

Quality Level

200

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Categorías relacionadas

Biochemicals

Biological Buffers

Application

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Nitric oxide reversibly inhibits the epidermal growth factor receptor tyrosine kinase.

C Estrada et al.

The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)

Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor

Hydrogen ion buffers for biological research.

Good NE, et al.

BioChemistry: An Indian Journal, 5, 467-477 (1996)

Direct determination of free triiodothyronine (T3) in undiluted serum by equilibrium dialysis/radioimmunoassay (RIA).

I J Chopra et al.

Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)

We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC

Direct biomolecule binding on nonfouling surfaces via newly discovered supramolecular self-assembly of lysozyme under physiological conditions.

Peng Yang

Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)

When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"

pH-dependent inhibition of native GABA(A) receptors by HEPES.

S Hugel et al.

British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)

Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar

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