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Merck

H3784

HEPES sodium salt

BioPerformance Certified, suitable for cell culture, ≥99.0%

Sinónimos:

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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Fórmula empírica (notación de Hill):
C8H17N2NaO4S
Número CAS:
75277-39-3
Peso molecular:
260.29
NACRES:
NA.25
PubChem Substance ID:
24895595
UNSPSC Code:
12161700
EC Number:
278-169-7
MDL number:
MFCD00036463

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InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

grade

BioPerformance Certified

assay

≥99.0%

form

powder

storage condition

dry at room temperature

technique(s)

activity assay: suitable, cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

color

white

pH

10-12 (1 M in water)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 0.1 M, clear, colorless

density

1.19 g/cm3 at 20—25 °C, 1.21 g/cm3 at 20—25 °C

absorption

≤0.02 at 280 in H2O at 0.1 M, ≤0.04 at 260 in H2O at 0.1 M

suitability

suitable for component for culture media, suitable for molecular biology

application(s)

cell analysis
diagnostic assay manufacturing
general analytical
life science and biopharma

foreign activity

DNase, RNase, NICKase, protease, none detected

Quality Level

400

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General description

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer with a remarkable pH buffering capacity between 6.8 and 8.2 (pKa of 7.48 at 25°C). Widely recognized for its versatility, HEPES is extensively employed in cell biology, biochemical, and biological research. Notably, its neutrality towards metal ions makes it a preferred choice for solutions with metal ions, acting as a "non-coordinating buffer."

In cell culture, HEPES excels in maintaining stable physiological pH even amidst fluctuating carbon dioxide concentrations, outperforming bicarbonate buffers (NaHCO3). Moreover, HEPES showcases valuable properties in various biological and biochemical processes, such as isoelectric focusing for protein separation and minimal interference in DNA-restriction enzyme reactions, making it indispensable across diverse research applications, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, exceptional buffering capacity, and minimal interaction with molecules establish HEPES as a key tool in various research domains.

Application

HEPES sodium salt has been used:
  • to supplement the HSOF medium
  • as a buffer component of HCZB medium in egg and embryo culture
  • as a buffer component in cell culture

Features and Benefits

  • High-purity product for biochemical and biological research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
  • Suitable for Cell culture
  • Tested for Endotoxins and Total Aerobic Microbial Count
  • Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease

Physical form

Lyophilized, spray dried, or crystallized material.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000526493
0000526491
0000526492
0000526495
0000526492

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Ekaterina Isaac et al.
Theriogenology, 161, 273-284 (2020-12-29)
The growth of viable cattle embryos in culture to stages beyond the hatching blastocyst is of interest to developmental biologists wishing to understand developmental events beyond the first lineage decision, as well as for commercial applications, because a lengthening of
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International journal of molecular sciences, 21(5) (2020-03-14)
Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to
Mingyue Li et al.
Structure (London, England : 1993), 27(5), 806-815 (2019-03-19)
The peroxidation of cardiolipins by reactive oxygen species, which is regulated and enhanced by cytochrome c (cyt c), is a critical signaling event in mitochondrial apoptosis. We probe the molecular underpinnings of this mitochondrial death signal through structural and functional
Artem Babaian et al.
Cell reports, 31(5), 107611-107611 (2020-05-07)
The ribosome is an RNA-protein complex that is essential for translation in all domains of life. The structural and catalytic core of the ribosome is its ribosomal RNA (rRNA). While mutations in ribosomal protein (RP) genes are known drivers of
Jihye Park et al.
The Analyst, 137(19), 4411-4414 (2012-08-07)
We have developed a quickly responsive, and specific fluorescent assay for the detection of Au(III) on the basis of the formation of gold nanoparticles in the presence of HEPES, which cleave the C-I bond of I-BODIPY 1 to yield the
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Product Information Sheet - H3784

Product Information Sheet

HEPES Sodium Salt Specification Comparison

HEPES Sodium Salt Specification Comparison

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