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Merck

H7033

PerfectHyb Plus Hybridization Buffer

for Northern and Southern blotting, solution

Sinónimos:

hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer

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NACRES:
NA.25
UNSPSC Code:
12161700
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Nombre del producto

PerfectHyb Plus Hybridization Buffer, for Northern and Southern blotting, solution

form

solution

storage temp.

room temp

Quality Level

Application

Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.

Features and Benefits

  • Fast and easy-to-use
  • Up to 20x more sensitive than conventional buffers
  • Doesn′t require blocking steps
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays
  • Compatible with radioactive and non-radioactive probes
  • Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes

General description

Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.

Other Notes

A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.

Legal Information

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Edyta Koscianska et al.
TheScientificWorldJournal, 11, 102-117 (2011-01-11)
This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists
Angel Emilio Martínez de Alba et al.
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In plants, most microRNAs (miRNAs) and several endogenous small interfering RNAs (siRNAs) bind to ARGONAUTE1 (AGO1) to regulate the expression of endogenous genes through post-transcriptional gene silencing (PTGS). AGO1 also participates in a siRNA-mediated PTGS defense response that thwarts exogenous
Yinping Jiao et al.
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miRNAs are known to play important regulatory roles throughout plant development. Until recently, nearly all the miRNAs in maize were identified by comparative analysis to miRNAs sequences of other plant species, such as rice and Arabidopsis. To find new miRNA
Shinpei Kawaoka et al.
RNA (New York, N.Y.), 15(7), 1258-1264 (2009-05-23)
Genetic studies and large-scale sequencing experiments have revealed that the PIWI subfamily proteins and PIWI-interacting RNAs (piRNAs) play an important role in germ line development and transposon control. Biochemical studies in vitro have greatly contributed to the understanding of small

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