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Merck

L0288

Lipid Mixture 1, Chemically Defined

liquid, sterile-filtered, BioReagent, suitable for cell culture

Sinónimos:

lipid mixture

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NACRES:
NA.75
UNSPSC Code:
12352211
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sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

storage temp.

2-8°C

Quality Level

Categorías relacionadas

General description

Lipid mixture 1, Chemically defined is recommended for use in cell culture at 1 to 10 mL per liter of medium.

Application

Lipid Mixture 1, Chemically Defined has been used:

  • as a component of bovine serum albumin (BSA) containing Bracket-Oliphant (BO) medium for the resuspension of sperm pellet
  • as a supplement in spheroid media for culturing primary human subcutaneous stromal vascular fraction cells
  • to prepare a cell steatosis model for culturing of mouse and human hepatocytes

Other Notes

Contains non-animal derived fatty acids (2 μg/ml arachidonic and 10 μg/ml each linoleic, linolenic, myristic, oleic, palmitic and stearic), 0.22 mg/ml cholesterol from New Zealand sheep′s wool, 2.2 mg/ml Tween-80, 70 μg/ml tocopherol acetate and 100 mg/ml Pluronic F-68 solubilized in cell culture water.
The final product contains 10% ethanol by volume.

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Juan Zhong et al.
International immunopharmacology, 59, 197-208 (2018-04-16)
The anti-inflammatory, immunomodulatory, and anticancer effects of micheliolide (MCL) isolated from Michelia champaca were previously reported, but its role and underlying mechanisms in relieving liver steatosis remain unclear. Herein, we investigated the effects of MCL on hepatic steatosis using a
Anna Ioannidou et al.
The Journal of physiology (2021-08-14)
Adipocyte enlargement is a key feature of obesity and associated with insulin resistance and metabolic disease The cause and consequences of adipocyte enlargement have remained hard to study in vitro due to a lack of human cell models with representative
Marc P Hübner et al.
PLoS neglected tropical diseases, 14(7), e0008427-e0008427 (2020-07-07)
A major impediment to eliminate lymphatic filariasis and onchocerciasis is the lack of effective short-course macrofilaricidal drugs or regimens that are proven to be safe for both infections. In this study we tested oxfendazole, an anthelmintic shown to be well
Md Mazharul Islam et al.
Reproductive medicine and biology, 20(3), 289-298 (2021-07-16)
The present study was undertaken to clarify whether bovine sperm could take up fatty acids (FAs) and produce ATP to maintain linear motility. Frozen bovine semen was thawed in media containing either lipid mixture (LM) or FAs, and sperm motility
Semir Beyaz et al.
Nature, 531(7592), 53-58 (2016-03-05)
Little is known about how pro-obesity diets regulate tissue stem and progenitor cell function. Here we show that high-fat diet (HFD)-induced obesity augments the numbers and function of Lgr5(+) intestinal stem cells of the mammalian intestine. Mechanistically, a HFD induces

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