L3892
Lectin from Triticum vulgaris (wheat)
peroxidase conjugate, lyophilized powder
Sinónimos:
WGA, Wheat germ agglutinin
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NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
≥85% protein basis (Warburg-Christian)
Biological source:
Triticum vulgaris
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Permítanos ayudarlebiological source
Triticum vulgaris
conjugate
peroxidase conjugate
assay
≥85% protein basis (Warburg-Christian)
form
lyophilized powder
potency
<40 μg/mL agglutination activity
peroxidase activity
50-200 units/mg protein
composition
Protein, ~90% modified Warburg-Christian
storage temp.
−20°C
Quality Level
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Application
Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Packaging
Package size based on protein content.
Physical form
Contains citrate buffer
Preparation Note
Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Other Notes
One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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