P4020
Protamine sulfate
amorphous powder
Sinónimos:
Salmine, Salmine from salmon
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Permítanos ayudarleNombre del producto
Protamine sulfate salt from salmon, Grade X, amorphous powder
type
Grade X
form
amorphous powder
impurities
histone, free (Millon test)
color
white to off-white
solubility
6 M HCl: 25 mg/mL
Quality Level
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Application
May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine sulfate salt from salmon has been used:
- in the pretreament of cells to study its effect on respiratory syncytial virus infection
- in lentivirus production
- to derivatize it into a diazeniumdiolate NO donor
Biochem/physiol Actions
Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.
General description
Protamine are proteins rich in cysteine and arginine. It possesses many phosphorylation sites. It is found to be localized to the sperm head.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Microarrays of lentiviruses for gene function screens in immortalized and primary cells
Bailey SN, et al.
Nature Methods, 3(2), 117-117 (2006)
The design and evaluation of heparin-binding foldamers.
Sungwook Choi et al.
Angewandte Chemie (International ed. in English), 44(41), 6685-6689 (2005-08-12)
Steve N Bailey et al.
Nature methods, 3(2), 117-122 (2006-01-25)
Here we describe lentivirus-infected cell microarrays for the high-throughput screening of gene function in mammalian cells. To create these arrays, we cultured mammalian cells on glass slides 'printed' with lentiviruses pseudotyped as vesicular stomatitis virus glycoprotein, which encode short hairpin
Diazeniumdiolation of protamine sulfate reverses mitogenic effects on smooth muscle cells and fibroblasts
Van Lith R, et al.
Free radical biology & medicine, 82, 13-21 (2015)
Packaging paternal chromosomes with protamine
Braun Robert E
Nature Genetics, 28(1), 10-10 (2001)
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