P4439
Phosphatase, Alkaline from porcine kidney
lyophilized powder, ≥100 DEA units/mg protein
Sinónimos:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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Número CAS:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
Specific activity:
≥100 DEA units/mg protein
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lyophilized powder
specific activity
≥100 DEA units/mg protein
composition
Protein, ≥50%
storage temp.
−20°C
Quality Level
Categorías relacionadas
Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.
Packaging
Package size based on DEA units
Physical form
Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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