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Merck

P8203

PIPES

BioXtra, ≥99% (titration)

Sinónimos:

1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

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About This Item

Fórmula empírica (notación de Hill):
C8H18N2O6S2
Número CAS:
5625-37-6
Peso molecular:
302.37
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24898917
EC Number:
227-057-6
Beilstein/REAXYS Number:
817713
MDL number:
MFCD00006159

Nombre del producto

PIPES, BioXtra, ≥99% (titration)

InChI key

IHPYMWDTONKSCO-UHFFFAOYSA-N

InChI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

SMILES string

OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O

product line

BioXtra

assay

≥99% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900°C)

≤0.5% (as SO4)

loss

≤0.5% loss on drying, 110°C

useful pH range

6.1-7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

solubility

1 M NaOH: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤0.2%

cation traces

Al: ≤0.005%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.005%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.1%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.1 at 260 in 1 M NaOH at 0.5 M
≤0.1 at 280 in 1 M NaOH at 0.5 M

application(s)

diagnostic assay manufacturing

Quality Level

400

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Other Notes

Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation.

Preparation Note

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

Analysis Note

Trace elemental analyses have been performed on the BioXtra PIPES; Certificate of Analysis provides lot-specific results. BioXtra PIPES is for applications which require tight control of elemental content.

Application

Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples.,
PIPES has been utilized in protein crystallization., The use of PIPES in the reconstitution of dissociated tubulin
α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8.
A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.

General description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000514185
0000514185
0000509903
0000509902
0000509902

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Y Zhou et al.
The Journal of biological chemistry, 275(10), 6975-6979 (2000-03-04)
The poor stability of membrane proteins in detergent solution is one of the main technical barriers to their structural and functional characterization. Here we describe a solution to this problem for diacylglycerol kinase (DGK), an integral membrane protein from Escherichia
S Haviernick et al.
Journal of microscopy, 135(Pt 1), 83-88 (1984-07-01)
It is suggested that the use of Hanks' + pipes + sucrose buffers, in combination with glutaraldehyde and osmium tetroxide fixatives, represent an excellent mode of preparation of fresh and cultured peripheral blood leucocytes, not only for transmission electron microscopy
Sambrook , J. and Russell, D.W.
Molecular Cloning: A Laboratory Manual, 7-7 (2001)
S A Moore et al.
Journal of molecular biology, 312(3), 511-523 (2001-09-21)
Human bile salt-stimulated lipase (BSSL), which is secreted from the pancreas into the digestive tract and from the lactating mammary gland into human milk, is important for the effective absorption of dietary lipids. The dependence of BSSL on bile acids
K E Loesser et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 34(11), 1477-1485 (1986-11-01)
We have modified the Lowicryl K4M low-temperature dehydration and embedding procedure for immunoelectron microscopy to provide improved ultrastructural detail and facilitate the localization of actin and tubulin in isolated rat adrenocortical cells, chick spinal cord with attached dorsal root ganglia
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