RDD002
HEPES
≥99.5%, crystalline powder, anhydrous, Redi-Dri™
Sinónimos:
Ácido 4-(2-hidroxietil)piperacina-1-etanosulfónico, Ácido N-(2-hidroxietil)piperacina-N′-(2-etanosulfónico)
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Fórmula empírica (notación de Hill):
C8H18N2O4S
Número CAS:
Peso molecular:
238.30
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
EC Number:
230-907-9
MDL number:
Beilstein/REAXYS Number:
883043
eCl@ss:
32129211
Servicio técnico
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Permítanos ayudarleNombre del producto
HEPES, anhydrous, free-flowing, Redi-Dri™, ≥99.5%
grade
anhydrous
Quality Level
product line
Redi-Dri™
assay
≥99.5%
form
crystalline powder
quality
free-flowing
pH
5.0-6.5 (25 °C, 238 g/L)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
water: 500 mg/mL, clear, colorless
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
General description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application
HEPES has been used:
- In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
- As a component in intracellular solution for the preparation of brain-slice recording
- As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
- To maintain the pH at 7 in thermal denaturation assay of purified protein
- In isocratic elution of protein using gel filtration
- Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy
Legal Information
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
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Clase de almacenamiento
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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