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Merck

S7635

Sulforhodamine 101

Dye content ~95 %, Powder

Sinónimos:

SR101; 2-(3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaen-16-yl)-5-sulfobenzenesulfonate

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About This Item

Fórmula empírica (notación de Hill):
C31H30N2O7S2
Número CAS:
60311-02-6
Peso molecular:
606.71
NACRES:
NA.47
PubChem Substance ID:
24899749
UNSPSC Code:
12171500
EC Number:
262-159-4
MDL number:
MFCD00012407
Beilstein/REAXYS Number:
3582548

Nombre del producto

Sulforhodamine 101, Dye content ~95 %

InChI

1S/C31H30N2O7S2/c34-41(35,36)20-9-10-21(26(17-20)42(37,38)39)27-24-15-18-5-1-11-32-13-3-7-22(28(18)32)30(24)40-31-23-8-4-14-33-12-2-6-19(29(23)33)16-25(27)31/h9-10,15-17H,1-8,11-14H2,(H-,34,35,36,37,38,39)

InChI key

COIVODZMVVUETJ-UHFFFAOYSA-N

SMILES string

OS(=O)(=O)c1ccc(C2=C3C=C4CCC[N+]5=C4C(CCC5)=C3Oc6c7CCCN8CCCc(cc26)c78)c(c1)S([O-])(=O)=O

form

powder

composition

Dye content, ~95%

technique(s)

microbe id | staining: suitable

color

black
dark green to brown

solubility

methanol: 1 mg/mL

ε (extinction coefficient)

≥105000 at 573-579 nm in ethanol

fluorescence

λex 586 nm; λem 605 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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Application

Sulforhodamine 101 has been used:
  • to label astrocytes
  • in quantitative polymerase chain reaction (PCR)
  • to determine the degree of disintegration in tissue sample

Biochem/physiol Actions

Sulforhodamine 101 can be specifically used as a marker of astroglia in the neocortex. It is commonly used for brain imaging. Studies suggest that sulforhodamine 101 may be used as an epileptogenic agent.

General description

Sulforhodamine 101 is a red fluorescent dye. It is water-soluble, amphoteric rhodamine.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
SHBR5977
SHBR9644
SHBR7199
SHBR0266
SHBP7695

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Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Nimmerjahn A, et al.
Nature Methods, 1(1), 31-31 (2004)
Florence Appaix et al.
PloS one, 7(4), e35169-e35169 (2012-04-18)
Fluorescent staining of astrocytes without damaging or interfering with normal brain functions is essential for intravital microscopy studies. Current methods involved either transgenic mice or local intracerebral injection of sulforhodamine 101. Transgenic rat models rarely exist, and in mice, a
Danielle Tokarz et al.
PloS one, 12(10), e0186846-e0186846 (2017-10-25)
Osteocytes are the most abundant cell in the bone, and have multiple functions including mechanosensing and regulation of bone remodeling activities. Since osteocytes are embedded in the bone matrix, their inaccessibility makes in vivo studies problematic. Therefore, a non-invasive technique
Jumpei Ukita et al.
Scientific reports, 9(1), 3791-3791 (2019-03-09)
A comprehensive understanding of the stimulus-response properties of individual neurons is necessary to crack the neural code of sensory cortices. However, a barrier to achieving this goal is the difficulty of analysing the nonlinearity of neuronal responses. Here, by incorporating
In vivo imaging of oligodendrocytes with sulforhodamine 101
Hill RA and Grutzendler J
Nature Methods, 11(11), 1081-1081 (2014)
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