SRP3136
PAF-AH human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Sinónimos:
1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, LDL-associated phospholipase A2, PAF 2-acylhydrolase, PAF acetylhydrolase
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Acerca de este artículo
NACRES:
NA.32
UNSPSC Code:
12352202
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Permítanos ayudarleNombre del producto
PAF-AH human, recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (HPLC)
≥95% (SDS-PAGE)
form
lyophilized
potency
≤0.1 ng/mL ED50
mol wt
47-55 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... PLA2G7(7941)
Biochem/physiol Actions
Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet activating factor acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the "sn2" ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL (low density lipoprotein) particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids. High levels of PAF-AH in plasma can be an indication of resistant hypertension. Polymorphism in PAF-AH gene at A379V might be linked with ischemic stroke susceptibility.
General description
Recombinant PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55kDa by SDS-PAGE analysis.
Other Notes
FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIALDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKER KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSNKASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN
Physical form
Lyophilized from 10 mM Tris, pH 8.5.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Association of PLA2G7 gene polymorphisms with ischemic stroke in northern Chinese Han population.
Liu X, et al.
Clinical Biochemistry, 47, 404-408 (2014)
Increased Lipoprotein-associated phospholipase A2 activity portends an increased risk of resistant hypertension.
Li Z, et al.
Lipids in Health and Disease, 15, 15-15 (2016)
Correlations between plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and PAF-AH genotype, age, and atherosclerosis in a Japanese population.
Yamada Y, et al.
Atherosclerosis, 150, 209-216 (2000)
Biology of platelet-activating factor acetylhydrolase (PAF-AH, lipoprotein associated phospholipase A2).
Stafforini DM
Cardiovascular Drugs and Therapy, 23, 73-83 (2009)
Hiroyuki Arai
Prostaglandins & other lipid mediators, 68-69, 83-94 (2002-11-16)
Platelet-activating factor (PAF) is one of the most potent lipid mediators and is involved in a variety of physiological events. The acetyl group at the sn-2 position of its glycerol backbone is required for its biological activity, and deacetylation of
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