T0440
3,3′,5,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, liquid
Sinónimos:
TMB
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About This Item
Nombre del producto
Sistema de sustrato líquido 3,3′,5,5′-tetrametilbencidina (TMB) para ELISA, peroxidase substrate
form
liquid
storage temp.
2-8°C
Quality Level
Categorías relacionadas
Application
Cómo utilizar nuestro sustrato TMB:
- Nuestro sustrato TMB se suministra como sustrato de peroxidasa listo para usar que contiene TMB en un tampón ligeramente ácido. Antes de la reacción con la peroxidasa, el sustrato debe ser una disolución de incolora a verde azulada clara. El sistema sustrato desarrolla un producto de reacción azul cuando reacciona con la peroxidasa.
- Una vez alcanzado el punto final del ensayo con la adición de ácido, se mide la intensidad del sustrato TMB a 450 nm.
- Nuestro sustrato TMB está recomendado para los procedimientos ELISA (micropocillo) y se ha utilizado en diversos ensayos como sustrato del conjugado de anticuerpos y peroxidasa de rábano picante para la detección de la glucoproteína del virus Hendra, la tetracosáctida, la inmunoglobulina A secretora (sIgA) y las trampas extracelulares de neutrófilos (NET).
- El sistema de sustrato líquido de 3,3′,5,5′-tetrametilbencidina (TMB) no se recomienda para aplicaciones de membrana. Dado que el sustrato TMB rinde un producto de reacción soluble, no se recomienda para histoquímica ni para inmunotransferencia.
General description
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Do I need to add hydrogen peroxide to Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
No hydrogen peroxide should be added to product T0440. The product, which contains a peroxidase substrate, already contains hydrogen peroxide. This solution is ready to use for ELISA assays.
What is the concentration of the TMB and what is the solvent in Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
The concentration of TMB in this formulation and the identity of the solvent are considered proprietary information. The product is a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer.
Do I need to dilute Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
No, the product should not be diluted.. The product is ready-to-use for ELISA applications. If the intensity of the reaction needs to be reduced, it is recommended that the antibodies or conjugates be diluted. Usage information is described on our data sheet.
What is the difference between products T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, and T8665, 3,3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System?
Both products are comparable and either one can be used in ELISA procedures. The formulations which are proprietary may have some slight differences.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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General Information
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