T3405

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

Name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride
Brand: SIGMA

chromogenic, tablet

Sinónimos:

4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

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About This Item

Fórmula empírica (notación de Hill):

C₁₆H₂₀N₂ · 2HCl

Número CAS:

64285-73-0

Peso molecular:

313.27

NACRES:

NA.83

PubChem Substance ID:

24900140

UNSPSC Code:

12352204

EC Number:

264-769-6

MDL number:

MFCD00012961

Nombre del producto

3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

InChI key

NYNRGZULARUZCC-UHFFFAOYSA-N

SMILES string

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

form

tablet

mp

≥300 °C

storage temp.

2-8°C

Quality Level

200

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Categorías relacionadas

Protein Biology

Proteins & Enzymes

Detection Substrates & Enzymes

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm.

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the

anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

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Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 40(1), 131-142 (2009-09-18)

Observational evidence suggests that infection with helminths protects against allergic disease and allergen skin sensitization. It is postulated that such effects are mediated by helminth-induced cytokine responses, in particular IL-10. We tested this hypothesis in a rural area of central

Nonhuman primate infants have an impaired respiratory but not systemic IgG antibody response following influenza virus infection.

Beth C Holbrook et al.

Virology, 476, 124-133 (2014-12-30)

Respiratory infection of young infants results in increased morbidity and mortality compared to infection of adults. In spite of the significance of this health issue, our understanding of the immune response elicited in infants especially in the respiratory tract is

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Current Alzheimer Research, 9(4), 397-405 (2012)

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Contenido relacionado

Product_Information_Sheet-T3405

Data Sheet - T3405

Instructions

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