T3824
Tirosinasa from mushroom
lyophilized powder, ≥1000 unit/mg solid
Sinónimos:
Catecoloxidasa, Monofenol monooxigenasa, Monofenol, dihidroxifenilalanina:oxígeno oxidorreductasa, Polifenol oxidasa
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Acerca de este artículo
biological source
fungus (mushroom)
form
lyophilized powder
specific activity
≥1000 unit/mg solid
mol wt
119.5 kDa by electrophoresis, 128 kDa by sedimentation velocity diffusion, 133 kDa by LS
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
Quality Level
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General description
El pH óptimo es 6-7
Peso molecular: 128 kDa por difusión de velocidad de sedimentación; 133 kDa mediante medición de dispersión de la luz y 119,5 kDa por electroforesis.
La tirosinasa es una oxidasa que contiene cobre, con actividad para catecoles y cresol. Es responsable de las reacciones de pardeamiento. Se ha comunicado que la enzima tiene dos sitios de unión para sustratos aromáticos y un sitio de unión diferente para oxígeno-cobre. El cobre es probablemente el Cu(I), y su inactivación implicará la oxidación a Cu(II).
El pH óptimo es 6-7
Peso molecular: 128 kDa por difusión de velocidad de sedimentación; 133 kDa mediante medición de dispersión de la luz y 119,5 kDa por electroforesis.
Application
Biochem/physiol Actions
Other Notes
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How can Tyrosinase from mushroom, Product T3824, be dissolved and what is its expected stability in solution?
Tyrosinase can be dissolved in 50 mM potassium phosphate buffer at 2 mg/mL. Solutions are expected to retain activity for several days in the refrigerator (2-8°C) and for several weeks in the freezer (-20°C).
The website says Product T3824, Tyrosinase from mushroom, should be stored at -20°C, but I received it at room temperature. Is this correct?
The product is shipped at ambient temperature and is stable for up to two weeks during shipment. Once received, it is recommended to store the product at -20°C to insure the quality for long term storage.
I see that the approximate activity of Product T3824, Tyrosinase from mushroom, is ?1000 unit/mg solid, but how can I find the lot specific activity of this tyrosinase?
The certificate of analysis for the lot will report the exact tyrosinase activity for a particular batch. The lot number can be found on the label of the bottle, or you can call technical service (800-325-5832) for a sample lot number to view the online certificate of analysis.
I used to order another tyrosinase (Product No. T7755), which has since been discontinued. What is the difference between the replacement product T3824 and the discontinued product T7755?
The difference between these two products is that T3824 has been stabilized with trehalose, while T7755 was not.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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