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Merck

T4648

Trombina from bovine plasma

lyophilized powder, 40-500 NIH units/mg protein (biuret)

Sinónimos:

Factor IIa

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About This Item

Número CAS:
9002-04-4
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-648-7
MDL number:
MFCD00166053
Número CE:
3.4.21.5 (BRENDA, IUBMB)

Nombre del producto

Trombina from bovine plasma, lyophilized powder, 40-500 NIH units/mg protein (biuret)

biological source

bovine plasma

form

lyophilized powder

specific activity

40-500 NIH units/mg protein (biuret)

mol wt

heavy chain ~33 kDa
light chain ~5 kDa

composition

Protein, 40-60%

Quality Level

300

Gene Information

cow ... F2(280685)

color

, white to faint yellow to faint red

UniProt accession no.

P00735

shipped in

wet ice

storage temp.

−20°C

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Analysis Note

En el procedimiento de análisis NIH se utilizan 0,2 ml de plasma diluido (1:1 con disolución salina) como sustrato y 0,1 ml de muestra de trombina (estabilizada en una disolución de albúmina tamponada al 1 %), basado en una modificación del método de Biggs. Para determinar las concentraciones de trombina sólo se utilizan los tiempos de coagulación comprendidos entre 15 y 25 segundos.
La actividad se expresa en unidades NIH obtenidas por comparación directa con el patrón de referencia de la trombina NIH, lote K.

Application

La trombina se utiliza para la rotura específica de sitio de las proteínas de fusión recombinantes que contienen un sitio de reconocimiento de trombina accesible para eliminar las etiquetas de afinidad. La trombina se ha utilizado en un estudio para evaluar la hipocoagulabilidad persistente en pacientes con choque séptico.

Biochem/physiol Actions

Serín proteasa que hidroliza selectivamente los enlaces Arg-Gly en el fibrinógeno para formar fibrina y fibrinopéptidos A y B.

General description

La trombina es la proteasa final de la coagulación con respecto a la hemostasia, promoviendo tanto efectos procoagulantes como anticoagulantes.

Other Notes

La actividad se expresa en unidades NIH obtenidas por comparación directa con un patrón de referencia de la trombina NIH.
Véase más información sobre la trombina en www.sigma-aldrich.com/enzymeexplorer.

Physical form

Polvo liofilizado que contiene cloruro de sodio y Tris-HCl, pH 7,0

Preparation Note

Se activa con tromboplastina cerebral bovina.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000517863
0000517849
0000517849
0000517863
0000339975

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solubility of Product T4648, Thrombin from bovine plasma?

    Thrombin from bovine plasma is soluble in water at 10 mg/mL.

  6. What is the solution stability of Product T4648, Thrombin from bovine plasma?

    When stock solutions of thrombin from bovine plasma are prepared at a concentration of 100 units/mL in 0.1% (w/v) BSA, they are stable for one week at 0-5°C.  Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store at -20°C for long term storage.

  7. What is the cross reactivity of Thrombin?

    Thrombin from any vertebrate species will clot the fibrinogen of virtually any other species.  Fibrinogen from any mammalian source will cross-react with thrombin from any mammalian source, but it is not well known how thrombin and fibrinogen from different animals differ in their sequence.  When any mammalian thrombin is injected into a different mammal, clotting will occur.

  8. What is the isoelectric point of Product T4648, Thrombin from bovine plasma?

    The isoelectric point of thrombin from bovine plasma is 7.05.

  9. How is the activity of Product T4648, Thrombin from bovine plasma, determined?

    The thrombin from bovine plasma activity is determined with a clotting assay (conversion of fibrinogen to fibrin) which measures alpha-thrombin and the amount of beta- and gamma-thrombin present is not measured. The assay of the clotting activity of thrombin from bovine plasma is performed at pH 7, utilizing a NIH thrombin reference standard.

  10. For thrombin, how do NIH units compare to International Units (IU)?

    One NIH unit of thrombin is equivalent to approximately 1.8 International Unit (IU) of thrombin.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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