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Fórmula empírica (notación de Hill):
C14H21ClN2O3S · HCl
Número CAS:
Peso molecular:
369.31
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352202
EC Number:
224-266-4
MDL number:
Beilstein/REAXYS Number:
7106867
eCl@ss:
32160406
Servicio técnico
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Permítanos ayudarleNombre del producto
Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride, ≥96% (TLC), powder
biological source
synthetic (organic)
Quality Level
assay
≥96% (TLC)
form
powder
potency
10-100 μM effective concentration
solubility
H2O: 50 mg/mL
storage temp.
−20°C
SMILES string
Cl[H].Cc1ccc(cc1)S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl
InChI
1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1
InChI key
YFCUZWYIPBUQBD-ZOWNYOTGSA-N
Gene Information
human ... NFKB1(4790), NFKB2(4791)
Application
Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used:
- in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extract
- as a protease inhibitor in tissue and cell homogenization
- as a trypsin inhibitor to determine the specific protease activity levels
Biochem/physiol Actions
Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Disclaimer
Stable at least two years when stored desiccated at −20°C.
Clase de almacenamiento
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1B
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