Saltar al contenido
Merck
  • Glutamate receptor activation evokes calpain-mediated degradation of Sp3 and Sp4, the prominent Sp-family transcription factors in neurons.

Glutamate receptor activation evokes calpain-mediated degradation of Sp3 and Sp4, the prominent Sp-family transcription factors in neurons.

Journal of neurochemistry (2007-02-24)
Xianrong Mao, Shao-Hua Yang, James W Simpkins, Steven W Barger
RESUMEN

Sp-family transcription factors (Sp1, Sp3 and Sp4) contain a zinc-finger domain that binds to DNA sequences rich in G-C/T. As assayed by RT-PCR analysis of mRNA, western-blot analysis, immunofluorescence, and antibody-dependent "supershift" of DNA-binding assays, the prominent Sp-family factors in cerebral neurons were identified as Sp3 and Sp4. By contrast, glial cells were found to express Sp1 and Sp3. We previously showed that the pattern of G-C/T binding activity of Sp-family factors is rapidly and specifically altered by the calcium influx accompanying activation of glutamate receptors. Here, we demonstrate that Sp-factor activity is also lost after a cerebral ischemia/reperfusion injury in vivo. Consistent with its calcium-dependent nature, we found that glutamate's effect on Sp-family factors could be blocked by inhibitors of calpains, neutral cysteine proteases activated by calcium. Purified calpain I cleaved Sp3 and Sp4 into products that retained G-C/T-binding activity, consistent with species observed in glutamate-treated neurons. These data provide details of an impact of glutamate-receptor activation on molecular events connected to gene expression.

MATERIALES
Product Number
Marca
Descripción del producto

Sigma-Aldrich
Anticuerpo anti-NeuN, clon A60, clone A60, Chemicon®, from mouse
Sigma-Aldrich
Caspase Inhibitor I, Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.
Sigma-Aldrich
Calpain Inhibitor III, The Calpain Inhibitor III, also referenced under CAS 88191-84-8, controls the biological activity of Calpain. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
Sigma-Aldrich
CA-074 Me, CA-074 Me, CAS 147859-80-1, is a cell-permeable analog of CA-074 that acts as an irreversible inhibitor of intracellular cathepsin B.
Sigma-Aldrich
Pepstatin A Methyl Ester, A cell-permeable methyl ester derivative of Pepstatin A that acts as a potent, non-competitive, transition-state analog inhibitor of γ-secretase.
Sigma-Aldrich
Calpain-1, Porcine Erythrocytes, Calpain-1, Porcine Erythrocytes, is a native calpain-1. A heterodimeric cysteine proteinase with low Ca2+ requirement (EC₅₀ = 2 µM).
Sigma-Aldrich
Cathepsin L Inhibitor II, The Cathepsin L Inhibitor II controls the biological activity of Cathepsin L. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
Sigma-Aldrich
ALLM, Cell permeable inhibitor of calpain I (Ki = 120 nM), calpain II (Ki = 230 nM), cathepsin B (Ki = 100 nM), and cathepsin L (Ki = 600 pM).
Sigma-Aldrich
Goat Anti-Rabbit IgG Antibody, FITC conjugate, 2 mg/mL, Chemicon®
Sigma-Aldrich
EST, A cell-permeable, irreversible inhibitor of cysteine proteases.
Sigma-Aldrich
Cathepsin L Inhibitor I, The Cathepsin L Inhibitor I, also referenced under CAS 108005-94-3, controls the biological activity of Cathepsin L. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
Sigma-Aldrich
Donkey Anti-Mouse IgG Antibody, Rhodamine conjugate, Species Adsorbed, Chemicon®, from donkey