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Merck

GCYT1-16K

MILLIPLEX® Chicken Cytokine/Chemokine Panel

Configurable Chicken Cytokine/Chemokine 12-Plex Panel

Synonym(s):

Avian inflammation assay, Luminex chicken cytokine multiplex assay, Millipore chicken cytokine kit, Poultry cytokine panel

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About This Item

NACRES:
NA.47
UNSPSC Code:
12161503

Product Name

MILLIPLEX® Chicken Cytokine/Chemokine Panel, Configurable Chicken Cytokine/Chemokine 12-Plex Panel

quality

100

species reactivity

chicken

manufacturer/tradename

Milliplex®

packaging

1 ea of

assay range

accuracy: 87-100%
(using the overnight protocol)

accuracy: 95-100%
(using the 2 hour protocol)

standard curve range: 12.21-20,000 pg/mL
(for RANTES)

standard curve range: 122.1-500,000 pg/mL
(for IL-6)

standard curve range: 18.3-75,000 pg/mL
(for IFNγ)

standard curve range: 18.3-75,000 pg/mL
(for MIP-3α)

standard curve range: 24.4-100,000 pg/mL
(for IL-10)

standard curve range: 3.66-15,000 pg/mL
(for IFNα)

standard curve range: 36.6-150,000 pg/mL
(for IL-21)

standard curve range: 4.88-20,000 pg/mL
(for MIP-1β)

standard curve range: 4.88-20,000 pg/mL
(for VEGF)

standard curve range: 48.8-200,000 pg/mL
(for IL-2)

standard curve range: 7.32-30,000 pg/mL
(for IL-16)

standard curve range: 9.75-40,000 pg/mL
(for M-CSF)

inter-assay cv: <20%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

input

serum plasma (Cell culture, cell culture supernatants)

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

Analysis Note

The protocol recommends a 1:4 dilution of serum and plasma samples.

Biochem/physiol Actions

Cross Reactivity:
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Features and Benefits

  • Comprehensive 12-plex Cytokine Panel: Simultaneously quantify 12 critical immune mediators in a single assay, dramatically improving research efficiency over traditional single-analyte ELISAs.
  • Quality-Assured Performance: Each kit includes quality controls, optimized serum matrix, and verified cross-reactivity testing with negligible interference between analytes, ensuring reliable and reproducible results you can trust.
  • Advanced disease mechanism investigation: Explore inflammatory pathways in chicken models of infectious diseases, osteoarthritis, respiratory conditions, and metabolic disorders to develop targeted therapeutic interventions and improve poultry health outcomes.
  • Customizable analyte selection: Design your personalized multiplex kit by selecting only the specific cytokines and chemokines relevant to your research objectives, optimizing cost-effectiveness while maintaining assay performance and reducing unnecessary analyte measurements.

General description

Studying cytokines and chemokines in chickens is crucial for advancing our knowledge of avian immunology and the mechanisms underlying various diseases. By examining the roles and interactions of these signaling molecules, researchers can gain valuable insights into immune responses, inflammation, and disease resistance in poultry. This research not only enhances our understanding of chicken biology but also informs the development of effective vaccination strategies and management practices to improve poultry health and welfare.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 12 analytes in chicken serum, plasma, and cell culture supernatants.

Analytes included: Interferon alpha (IFNα), Interferon gamma (IFNγ), Interleukin 2 (IL-2), Interleukin 6 (IL-6), Interleukin 10 (IL-10), Interleukin 16 (IL-16), Interleukin 21 (IL-21), Macrophage Inflammatory protein-1β (MIP-1b), Macrophage Inflammatory protein-3 (MIP-3a), RANTES (regulated on activation, normal T cell expressed and secreted), Macrophage colony-stimulating factor (M-CSF), Vascular endothelial growth factor (VEGF)

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
GCYT1-16K-0704065265239592
GCYT1-16K-10C04065265239745
GCYT1-16K-03C04065265239677
GCYT1-16K-0404065265239561
GCYT1-16K-04C04065265239684
GCYT1-16K-08C04065265239721
GCYT1-16K-09C04065265239738
GCYT1-16K-0204065265239547
GCYT1-16K-1204065265239646
GCYT1-16K-01C04065265239653
GCYT1-16K-06C04065265239707
GCYT1-16K-0104065265239530
GCYT1-16K-1104065265239639
GCYT1-16K-07C04065265239714
GCYT1-16K-0904065265239615
GCYT1-16K-0304065265239554
GCYT1-16K-05C04065265239691
GCYT1-16K-0504065265239578
GCYT1-16K-0804065265239608
GCYT1-16K-02C04065265239660
GCYT1-16K-12C04065265239769
GCYT1-16K-1004065265239622
GCYT1-16K-0604065265239585
GCYT1-16K-11C04065265239752

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