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386041 Anti-Hsp90 Rat mAb (16F1)

Anti-Hsp90 Rat mAb (16F1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Hsp82, Anti-Hsp85, Anti-Hsp89

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      A Broad Range Of SpeciesRMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~90 kDa Hsp90 protein.
      Catalogue Number386041
      Brand Family Calbiochem®
      SynonymsAnti-Hsp82, Anti-Hsp85, Anti-Hsp89
      Application Data
      Detection of Hsp90 by immunoblotting. Samples: Molecular weight marker (lane 1), Hsp90 native protein (lane 2), Hsp90 recombinant protein (lane 3), Hsp90 alpha recombinant protein (lane 4), heat shocked HeLa (lane 5), L-929 (lane 6), Rat-2 cells (lane 7), and RK-13 cells (lane 8). Primary antibody: Anti-Hsp90 Rat mAb (16F1) (Cat. No. 386041) (1:1000). Detection: chemiluminescence.
      References
      ReferencesSchuh, S., et al. 1985. J. Biol. Chem. 260, 14292.
      Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.
      Lanks, K.W., et al. 1982. J. Biol. Chem. 257, 8604.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Positive controlHeat shocked HeLa cells, L929 cells, Rat-2 cells, RK-13 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunohistochemistry
      Immunoprecipitation
      Application NotesELISA (see comments)
      Immunoblotting (2 µg/ml, colormetric)
      Immunocytochemistry (see comments)
      Immunohistochemistry (see comments)
      Immunoprecipitation (see comments)
      Electron Microscopy (see comments)
      Application CommentsThis antibody also works in plants, specifically cotton, by immunoblot analysis. Has also been reported to work for immunoprecipitation, ELISA, immunohistochemistry, immunocytochemistry, and electron microscopy. Note: Rat immunoglobulins do not react well with protein A. For immunoprecipitation, protein G is recommended. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenHsp90 isolated from therapeutic human orchiectomy specimens
      ImmunogenHuman
      Clone16F1
      HostRat
      IsotypeIgG2a
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      386041 0

      Documentation

      Anti-Hsp90 Rat mAb (16F1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Hsp90 Rat mAb (16F1) Certificates of Analysis

      TitleLot Number
      386041

      References

      Reference overview
      Schuh, S., et al. 1985. J. Biol. Chem. 260, 14292.
      Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.
      Lanks, K.W., et al. 1982. J. Biol. Chem. 257, 8604.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-November-2011 JSW
      SynonymsAnti-Hsp82, Anti-Hsp85, Anti-Hsp89
      ApplicationELISA (see comments)
      Immunoblotting (2 µg/ml, colormetric)
      Immunocytochemistry (see comments)
      Immunohistochemistry (see comments)
      Immunoprecipitation (see comments)
      Electron Microscopy (see comments)
      Application Data
      Detection of Hsp90 by immunoblotting. Samples: Molecular weight marker (lane 1), Hsp90 native protein (lane 2), Hsp90 recombinant protein (lane 3), Hsp90 alpha recombinant protein (lane 4), heat shocked HeLa (lane 5), L-929 (lane 6), Rat-2 cells (lane 7), and RK-13 cells (lane 8). Primary antibody: Anti-Hsp90 Rat mAb (16F1) (Cat. No. 386041) (1:1000). Detection: chemiluminescence.
      DescriptionProtein G purified rat monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
      BackgroundHsp90 (heat shock protein 90) is a primarily cytoplasmic protein which exists in dimeric form. Hsp90 binds several other cellular proteins such as retrovirus kinases (pp60v-src), steroid receptors, heme-regulated protein kinase, actin, and tubulin. Although Hsp90 expression is induced by stress, and thus it may be an indicator of cell stress, its constitutive level may be as high as 1-2% of the total extractable cellular protein.
      HostRat
      Immunogen speciesHuman
      ImmunogenHsp90 isolated from therapeutic human orchiectomy specimens
      Clone16F1
      IsotypeIgG2a
      Speciesa broad range of species
      Positive controlHeat shocked HeLa cells, L929 cells, Rat-2 cells, RK-13 cells
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody also works in plants, specifically cotton, by immunoblot analysis. Has also been reported to work for immunoprecipitation, ELISA, immunohistochemistry, immunocytochemistry, and electron microscopy. Note: Rat immunoglobulins do not react well with protein A. For immunoprecipitation, protein G is recommended. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSchuh, S., et al. 1985. J. Biol. Chem. 260, 14292.
      Lai, B.-T., et al. 1984. Mol. Cell. Biol. 4, 2802.
      Lanks, K.W., et al. 1982. J. Biol. Chem. 257, 8604.