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420092 PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb

This PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb is validated for use in Immunoblotting for the detection of JAK1 (pTyr1022/1023).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, MRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~130 kDa phosphorylated JAK1 in LIF-treated 3T3-L1 cells. Does not cross-react with other JAKs or related kinases.
      Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
      Catalogue Number420092
      Brand Family Calbiochem®
      Application Data
      Detection of mouse JAK1, phosphorylated on Tyr1022/1023, by immunoblotting. Samples: Whole cell lysate from 3T3L1 cells left unstimulated (lane 1) or stimulated with LIF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb (Cat. No. 420092) (1:1000) pre-incubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      References
      ReferencesLiu, K.D., et al. 1997. Curr. Biol. 7, 817.
      Gauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.
      Product Information
      DeclarationManufactured by Biosource International.
      FormLiquid
      FormulationIn Dulbecco's PBS (without Ca2+ and Mg2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Positive control3T3L1 cells treated with LIF
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsA ~110 kDa degradation product is also visible in some cell or tissue preparations. Does not cross-react with any other JAK proteins. Immunogen sequence is conserved in pig JAK1, but cross-reactivity has not been tested. When used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the optimal dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰²²and Tyr¹⁰²³ phosphorylation sites of human JAK1 (Janus Kinase 1)
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing intitial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      420092 0

      Documentation

      PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb Certificates of Analysis

      TitleLot Number
      420092

      References

      Reference overview
      Liu, K.D., et al. 1997. Curr. Biol. 7, 817.
      Gauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-May-2013 JSW
      ApplicationImmunoblotting (1:1000)
      Application Data
      Detection of mouse JAK1, phosphorylated on Tyr1022/1023, by immunoblotting. Samples: Whole cell lysate from 3T3L1 cells left unstimulated (lane 1) or stimulated with LIF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb (Cat. No. 420092) (1:1000) pre-incubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK1 protein phosphorylated on Tyr1022/1023.
      BackgroundIn response to cytokines or related factors (interferons, interleukins, etc.), JAKs are activated via phosphorylation at 2 adjacent tyrosine residues, as well as perhaps one or more serine residues. Activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activators of transcription) proteins, which dimerize and translocate to the nucleus. JAK1 is required for signal transduction through IL-2, IL-4, IL-6, GM-CSF, and interferon receptors.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰²²and Tyr¹⁰²³ phosphorylation sites of human JAK1 (Janus Kinase 1)
      IsotypeIgG
      Specieshuman, mouse, not rat
      Positive control3T3L1 cells treated with LIF
      FormLiquid
      FormulationIn Dulbecco's PBS (without Ca2+ and Mg2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Preservative≤0.1% sodium azide
      CommentsA ~110 kDa degradation product is also visible in some cell or tissue preparations. Does not cross-react with any other JAK proteins. Immunogen sequence is conserved in pig JAK1, but cross-reactivity has not been tested. When used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the optimal dilution.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing intitial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesLiu, K.D., et al. 1997. Curr. Biol. 7, 817.
      Gauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.