Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Description
Overview
Recombinant, human neuregulin 1-β1/heregulin 1-β1 extracellular domain (NRG1-beta1/HRG1-beta1 ECD) consisting of amino acids 2-246 and expressed in E. coli. All NRG1 isoforms contain an EGF-like domain (β-splice variant) that is used to directly bind to the ErbB3 or ErbB4 receptor tyrosine kinases.
Catalogue Number
492028
Brand Family
Calbiochem®
References
References
Buonanno, A., and G.D Fischbach 2001. Curr. Opin. Neurobiol.11, 287. Adlkofer, K. and C. Lai 2000. Glia29, 104. Garratt, A.N. et al., 2000. BioEssays22, 987. Karey, K.P., et al. 1988. Cancer Res.48, 4083.
Product Information
Form
Lyophilized
Formulation
Lyophilized from 0.2 µm filtered acetonitrile, TFA, BSA.
ED₅₀ = 2.5-12.5 ng/ml as measured in a serum-free proliferation assay using MCF7 human breast adenocarcinoma cells.
Purity
≥90% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short term-storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
NRG1-β1/HRG1-β1 ECD, Human, Recombinant Certificates of Analysis
Title
Lot Number
492028
References
Reference overview
Buonanno, A., and G.D Fischbach 2001. Curr. Opin. Neurobiol.11, 287. Adlkofer, K. and C. Lai 2000. Glia29, 104. Garratt, A.N. et al., 2000. BioEssays22, 987. Karey, K.P., et al. 1988. Cancer Res.48, 4083.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
22-June-2011 RFH
Description
Recombinant, human neuregulin 1-β1/heregulin 1-β1 extracellular domain (NRG1-beta1/HRG1-beta1 ECD) consisting of amino acids 2-246 and expressed in E. coli. All NRG1 isoforms contain an EGF-like domain (β-splice variant) that is used to directly bind to the ErbB3 or ErbB4 receptor tyrosine kinases.
Form
Lyophilized
Formulation
Lyophilized from 0.2 µm filtered acetonitrile, TFA, BSA.
Purity
≥90% by SDS-PAGE
Contaminants
Endotoxin: ≤1.0 EU/µg protein
Biological activity
ED₅₀ = 2.5-12.5 ng/ml as measured in a serum-free proliferation assay using MCF7 human breast adenocarcinoma cells.
Solubility
Reconstitute 50 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution refrigerate (4°C) for short term-storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Buonanno, A., and G.D Fischbach 2001. Curr. Opin. Neurobiol.11, 287. Adlkofer, K. and C. Lai 2000. Glia29, 104. Garratt, A.N. et al., 2000. BioEssays22, 987. Karey, K.P., et al. 1988. Cancer Res.48, 4083.
