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616378 Anti-TRAIL, C-Terminal (261-277) Rabbit pAb

This Anti-TRAIL, C-Terminal (261-277) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of TRAIL, C-Terminal (261-277).

Anti-TRAIL, C-Terminal (261-277) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TNF-related Apoptosis-inducing Ligand, Anti-APO2L

616378
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      OverviewRecognizes the ~32 kDa TRAIL protein in HeLa cells.

      This product has been discontinued.
      Catalogue Number616378
      Brand Family Calbiochem®
      SynonymsAnti-TNF-related Apoptosis-inducing Ligand, Anti-APO2L
      References
      ReferencesPan, G., et al. 1997. Science 277, 815.
      Schneider, P., et al. 1997. Immunity 7, 831.
      Sheridan, J.P., et al. 1997. Science 277, 818.
      Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
      Wiley, S.R., et al. 1995. Immunity 3, 673.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHeLa cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1:500-1:1000)
      Application CommentsAlso exhibits slight cross-reactivity with a protein at ~85 kDa in HeLa cell lysates. This antibody is also reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (TNEHLIDMDHEASFFGA) corresponding to amino acids 261-277 of human TRAIL, conjugated to carrier protein
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      616378 0

      Documentation

      Anti-TRAIL, C-Terminal (261-277) Rabbit pAb Certificates of Analysis

      TitleLot Number
      616378

      References

      Reference overview
      Pan, G., et al. 1997. Science 277, 815.
      Schneider, P., et al. 1997. Immunity 7, 831.
      Sheridan, J.P., et al. 1997. Science 277, 818.
      Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
      Wiley, S.R., et al. 1995. Immunity 3, 673.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-August-2007 RFH
      SynonymsAnti-TNF-related Apoptosis-inducing Ligand, Anti-APO2L
      ApplicationELISA (see comments)
      Immunoblotting (1:500-1:1000)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~32 kDa TRAIL protein.
      BackgroundTRAIL is a type II membrane protein expressed in a variety of human tissues. Two novel death domain-containing receptors, DR4 and DR5, have been identified as the receptor for TRAIL. Like TNF and Fas ligand, TRAIL induces apoptosis and NFκB activation in many tissues and cells.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (TNEHLIDMDHEASFFGA) corresponding to amino acids 261-277 of human TRAIL, conjugated to carrier protein
      IsotypeIgG
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAlso exhibits slight cross-reactivity with a protein at ~85 kDa in HeLa cell lysates. This antibody is also reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesPan, G., et al. 1997. Science 277, 815.
      Schneider, P., et al. 1997. Immunity 7, 831.
      Sheridan, J.P., et al. 1997. Science 277, 818.
      Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
      Wiley, S.R., et al. 1995. Immunity 3, 673.