Millipore Sigma Vibrant Logo

616465 Anti-Transglutaminase II Rabbit pAb

Anti-Transglutaminase II Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
616465
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~77-85 kDa transglutaminase II protein.
      Catalogue Number616465
      Brand Family Calbiochem®
      Application Data
      Detection of rat transglutaminase II by immunohistochemistry. Sample: Rat spleen. Primary antibody: Anti-Transglutaminase II Rabbit pAb (Cat. No. 616465) (5 µg/ml). Detection: fluorescence.

      Detection of human transglutaminase II by immunoblotting. Sample: Human transglutaminase II. Primary antibody: Anti-Transglutaminase II Rabbit pAb (Cat. No. 616465) (5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesDe Laurenzi, V., and Melino, G. 2001. Mol. Cell. Biol. 21, 148.
      Nanda, N., et al. 2001. J. Biol. Chem. 276, 20673.
      Kim, S.Y., and Grant, P.l. 1999. J. Biol. Chem. 274, 30715.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (5-10 µg/ml)
      Immunoblotting (5 µg/ml)
      Immunoprecipitation (10 µg/mg protein from lysate)
      Paraffin Sections (5-10 µg/ml, heat pre-treatment required)
      Application CommentsStaining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Does not cross-react with Factor XIIIa, keratinocyte TGase I, fibrinogen, fibronectin, collagen, or actin. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogenfull-length, recombinant, human cellular/tissue transglutaminase II
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      616465 0

      Documentation

      Anti-Transglutaminase II Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Transglutaminase II Rabbit pAb Certificates of Analysis

      TitleLot Number
      616465

      References

      Reference overview
      De Laurenzi, V., and Melino, G. 2001. Mol. Cell. Biol. 21, 148.
      Nanda, N., et al. 2001. J. Biol. Chem. 276, 20673.
      Kim, S.Y., and Grant, P.l. 1999. J. Biol. Chem. 274, 30715.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-August-2007 RFH
      ApplicationFrozen Sections (5-10 µg/ml)
      Immunoblotting (5 µg/ml)
      Immunoprecipitation (10 µg/mg protein from lysate)
      Paraffin Sections (5-10 µg/ml, heat pre-treatment required)
      Application Data
      Detection of rat transglutaminase II by immunohistochemistry. Sample: Rat spleen. Primary antibody: Anti-Transglutaminase II Rabbit pAb (Cat. No. 616465) (5 µg/ml). Detection: fluorescence.

      Detection of human transglutaminase II by immunoblotting. Sample: Human transglutaminase II. Primary antibody: Anti-Transglutaminase II Rabbit pAb (Cat. No. 616465) (5 µg/ml). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~77-85 kDa transglutaminase II protein.
      BackgroundTransglutaminases are a family of enzymes that catalyze calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. Transglutaminase II has been implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.
      HostRabbit
      Immunogen speciesHuman
      Immunogenfull-length, recombinant, human cellular/tissue transglutaminase II
      IsotypeIgG
      Specieshuman, mouse, rat
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsStaining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Does not cross-react with Factor XIIIa, keratinocyte TGase I, fibrinogen, fibronectin, collagen, or actin. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesDe Laurenzi, V., and Melino, G. 2001. Mol. Cell. Biol. 21, 148.
      Nanda, N., et al. 2001. J. Biol. Chem. 276, 20673.
      Kim, S.Y., and Grant, P.l. 1999. J. Biol. Chem. 274, 30715.