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AB7356-25UG Rb X vWF

This Anti-von Willebrand Factor Antibody is validated for use in ELISA, Immunohistochemistry (IHC) for the detection of von Willebrand Factor.

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AB7356-25UG
25 μg  
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      Description
      Catalogue NumberAB7356-25UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionRb X vWF
      Alternate Names
      • Factor VIII Related Antigen
      Background Informationvon Willebrand Factor (vWF) is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. It is one of the most widely used endothelial cell markers for studying angiogenesis / neovascularization.
      Millipore's von Willebrand Factor antibody reacts specifically with the endothelial cells of blood vessels and is a useful marker for the identification of endothelial lineage in tumors.
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL: Human tonsils, Mouse and rat brain.
      PresentationPurified rabbit immunoglobulin in buffer containing 0.02 M PBS pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-von Willebrand Factor Antibody is validated for use in ELISA, Immunohistochemistry (IHC) for the detection of von Willebrand Factor.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      Application NotesELISA:
      A 1:1000-1:5000 dilution of a previous lot was used in direct ELISA.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman von Willebrand Factor purified from plasma.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityAB7356 is specific for vWF by ELISA against the human protein and by immunohistochemistry. The antibody is reactive in paraffin embedded tissues on human, rat, and mouse. Other species not tested. Antibody has not been evaluated by western blot analysis for reactivity to vWF.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22.
      Gene Symbol
      • VWF
      • F8VWF
      • F8
      • vWF
      • VWD
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04275 # Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
      SIZE: 2813 amino acids; 309299 Da
      SUBUNIT: Multimeric. Interacts with F8.
      SUBCELLULAR LOCATION: Secreted. Note=Localized to storage granules.
      TISSUE SPECIFICITY: Plasma.
      DOMAIN: SwissProt: P04275 The von Willebrand antigen 2 is required for multimerization of VWF and for its targeting to storage granules.
      PTM: All cysteine residues are involved in intrachain or interchain disulfide bonds.
      DISEASE: SwissProt: P04275 # Defects in VWF are associated with various forms of von Willebrand disease (VWD) [MIM:193400, 277480]. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.). Type I VWD is associated with a deficiency of VWF; type II by normal to decreased plasma level of VWF; type III by a virtual absence of VWF. There are subtypes (A to H) of type II VWD; for example: type IIA is characterized by the absence of VWF high molecular weight multimers in plasma.
      SIMILARITY: Contains 1 CTCK (C-terminal cystine knot-like) domain. & Contains 4 TIL (trypsin inhibitory-like) domains. & Contains 3 VWFA domains. & Contains 3 VWFC domains. & Contains 4 VWFD domains.
      Molecular Weight250 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunohistochemistry(paraffin):
      Representative images from a previous lot.
      Rabbit anti-vWF (AB7356) staining of Human Cerebral Cortex. Tissue pretreated with Citrate Buffer, ph 6.0, anti-vWF at 20 μg/mL, IHC-Select Detection with HRP-DAB.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB7356-25UG 04054839350313

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