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AB9018-50UL Anti-Muscarinic Acetylcholine Receptor m3 Antibody

This Anti-Muscarinic Acetylcholine Receptor m3 Antibody is validated for use in WB for the detection of Muscarinic Acetylcholine Receptor m3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: CHRM3, HM3 UniProt Number: P20309
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AB9018-50UL
50 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9018-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Muscarinic Acetylcholine Receptor m3 Antibody
      References
      Product Information
      FormatAffinity Purified
      Control
      • Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Muscarinic Acetylcholine Receptor m3 Antibody is validated for use in WB for the detection of Muscarinic Acetylcholine Receptor m3.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:2000 using ECL on rat brain lysate.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.

      SUGGESTED WESTERN BLOT PROTOCOL

      1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.

      2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.

      3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)

      4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).

      5. Destain with deionized water.

      6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).

      7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.

      8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.

      9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.

      10. Wash 4 x 10 min. with PBS-0.1% tween 20.

      11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600).
      Biological Information
      ImmunogenSynthetic peptide from the 3rd intracellular loop of rat m3 (Accession P08483). The immunogen sequence is identical in human, mouse, bovine and porcine.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityMuscarinic acetylcholine receptor m3.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue.
      Gene Symbol
      • CHRM3
      • HM3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20309 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
      SIZE: 590 amino acids; 66128 Da
      SUBCELLULAR LOCATION: Basolateral cell membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: P20309 ## Belongs to the G-protein coupled receptor 1 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB9018-50UL 04053252330940

      Documentation

      Anti-Muscarinic Acetylcholine Receptor m3 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Muscarinic Acetylcholine Receptor m3 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-MUSCARINIC ACETYLCHOLINE RECEPTOR M3 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2153008 2153008
      RABBIT ANTI-MUSCARINIC ACETYLCHOLINE RECEPTOR M3 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2381741 2381741
      RABBIT ANTI-MUSCARINIC ACETYLCHOLINE RECEPTOR M3 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2426498 2426498
      RABBIT ANTI-MUSCARINIC ACETYLCHOLINE RECEPTOR M3 - 3886012 3886012
      RABBIT ANTI-MUSCARINIC ACETYLCHOLINE RECEPTOR M3 -2661306 2661306
      RABBIT ANTI-MUSCARINIC ACETYLCHOLINE RECEPTOR M3 -2782375 2782375
      RABBIT ANTI-MUSCARINIC ACETYLCHOLINE RECEPTOR M3 -2820260 2820260
      RABBIT ANTI-MUSCARINIC ACETYLCHOLINE RECEPTOR M3 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3078417
      RABBIT ANTI-MUSCARINIC ACETYLCHOLINE RECEPTOR M3 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2928710
      RABBIT ANTI-MUSCARINIC ACETYLCHOLINE RECEPTOR M3 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2820260A
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