Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Anti-monomethyl-Histone H3 (Lys36) Antibody Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated at lysine 36. Also known as Anti-H3K36me1, this antibody has dot blot (DB) proven specificity & has been validated in WB, IP. Small pack size is ideal for screening applications.
More>>Anti-monomethyl-Histone H3 (Lys36) Antibody Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated at lysine 36. Also known as Anti-H3K36me1, this antibody has dot blot (DB) proven specificity & has been validated in WB, IP. Small pack size is ideal for screening applications. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Histone H3 is one of four histones that form a core octameric structure that is required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can alter chromatin structure and stability, and influence DNA repair and gene expression. Histone H3 undergoes methylation of its lysine and arginine residues, with varying biological outcomes. The lysine 36 residue undergoes mono-, di-, or trimethylation which may be performed by one of many methyltransferase enzymes (SET2, NSD1, NSD2, NSD3, ASH1, SMYD2, SETMAR, KMT3A, and SETD3). Several studies have indicated that methylated lysine 36 residues promote Rpd3S-mediated deacetylation of other histones resulting in gene activation.
References
Product Information
Format
Affinity Purified
Control
HeLa acid extract and various recombinant Histone proteins
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-monomethyl-Histone H3 (Lys36) Antibody Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated at lysine 36. Also known as Anti-H3K36me1, this antibody has dot blot (DB) proven specificity & has been validated in WB, IP. Small pack size is ideal for screening applications.
Key Applications
Western Blotting
Immunoprecipitation
Dot Blot
Application Notes
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl-Histone H3 (Lys36) from HeLa acid extract.
Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see table) were probed with Anti-monomethyl-Histone H3 (Lys36) (0.5 µg/mL).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 monomethylated at Lys36.
Epitope
Monomethylated Lys36
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the N-terminus of Histone H3 monomethylated at Lys36.
Evaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.
Western Blot Analysis: 0.05 µg/mL of this antibody detected monomethyl-Histone H3 (Lys36) in 10 µg of HeLa acid extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
