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ABT1373-25UL Anti-Aggrecan

Anti-Aggrecan, Cat. No. ABT1373, is a rabbit polyclonal antibody that detects Aggrecan core protein and is tested for use in Immunohistochemistry, Immunohistochemistry (Paraffin), Radioimmunoassay, and Western Blotting.

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ABT1373-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABT1373-25UL
      DescriptionAnti-Aggrecan
      Alternate Names
      • Aggrecan core protein
      • Cartilage-specific proteoglycan core protein
      • CSPCP
      Background InformationAggrecan core protein (UniProt: P07897; also known as Cartilage-specific proteoglycan core protein, CSPCP) is encoded by the Acan (also known as Agc, Agc1) gene (GeneID: 58968) in rat. Aggrecan is a secreted proteoglycan that is a major component of extracellular matrix of cartilagenous tissues. Its major function is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region and may play a regulatory role in the matrix assembly of the cartilage. Aggrecan contains two globular domains, G1 and G2 that comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. The G1 region is located in the N-terminus of the core protein and interacts with hyaluronic acid (HA). G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. The G2 region is composed of two B-like domains, but does not have the ability to interact with HA. G2 region is separated from the G1 region by the interglobular domain, which is a prominent site for proteolysis. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3. Aggrecan is present in the extracellular matrix in the form of proteoglycan aggregates, in which many aggrecan molecules interact with hyaluronan and a link protein stabilizes each interaction. It is reported that aggrecan structure changes throughout the life cycle due to changes in synthetic and degradative events. Changes due to synthesis alter the structure of the chondroitin sulfate and keratan sulfate chains, whereas those due to degradation cause cleavage of all components of the aggregate. Aggrecan is also present in most of the retina including the outer nuclear layer, the outer plexiform layer, and the inner plexiform layer.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Applications
      ApplicationAnti-Aggrecan, Cat. No. ABT1373, is a rabbit polyclonal antibody that detects Aggrecan core protein and is tested for use in Immunohistochemistry, Immunohistochemistry (Paraffin), Radioimmunoassay, and Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Radioimmunoassay
      • Western Blotting
      Application NotesRadioimmunoassay Analysis: A representative lot detected Aggrecan in Postnatal rat brain (Milev, P., et. al. (1998). Biochem Biopys Res Commun. 247(2):207-12).

      Immunohistochemistry Analysis: A representative lot detected Aggrecan in Embryonic day 16 rat eye, P0, P9 and adult rat retina (Popp, S., et. al. (2004). Exp Eye Res. 79(3):351-6) and Embryonic day 16, 19 rat brain and postnatal day 7 cerebellum, embryonic day 19 spinal cord (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).

      Western Blotting Analysis: A representative lot detected Aggrecan in purified proteoglycans from a PBS extract of 7-day rat brain with chondroitinase ABC treatment (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 213 amino acids from the C-terminal half of rat Aggrecan.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects aggrecan in rat eye sections.
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteRat.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • Acan
      • Agc
      • Agc1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight221.12 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat retina tissue.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Aggrecan in rat retina tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT1373-25UL 04054839512445

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