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AM65 Anti-Caspase-3 (Ab-4) Mouse mAb (AM1.31-11)

Anti-Caspase-3 (Ab-4), mouse monoclonal, clone AM1.31-11, recognizes the ~11 kDa active subunit of caspase-3. It is validated for Western blotting and for use with paraffin sections.

Anti-Caspase-3 (Ab-4) Mouse mAb (AM1.31-11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Apopain, Anti-CASP3, Anti-CPP32, Anti-ICE-3, Anti-LICE, Anti-YAMA

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AM65
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, MMMonoclonal Antibody
      Description
      OverviewRecognizes the ~11 kDa active subunit of caspase-3.
      Catalogue NumberAM65
      Brand Family Calbiochem®
      SynonymsAnti-Apopain, Anti-CASP3, Anti-CPP32, Anti-ICE-3, Anti-LICE, Anti-YAMA
      Application Data
      Detection of human caspase-3 by staining paraffin sections. Sample: Breast carcinoma tissue, paraffin-embedded. Primary antibody: Anti-Caspase-3 (Ab-4) Mouse mAb (AM1.31-11) (Cat. No. AM65) (2.5 µg/ml). Detection: DAB
      References
      ReferencesAlnemri, E.S. 1997. J. Cell. Biochem. 64, 33.
      Bayaert, R., et al. 1997. J. Biol. Chem. 272, 11694.
      Erhardt, P., et al. 1997. J. Biol. Chem. 272, 15049.
      Inayat-Hussain, S.H., et al. 1997 Hepatology 25, 1516.
      Krjewska, M., et al. 1997. Cancer Res. 57, 1605.
      Mashima, T., et al. 1997. Oncogene 14, 1007.
      McConnell, K.R., et al. 1997. J. Immunol. 158, 2083.
      Posmantur, R., et al. 1997. J. Neurochem. 68, 2328.
      Suzuki, A., et al. 1997. Exp. Cell. Res. 233, 48.
      Liu, X., et al. 1996. J. Biol. Chem. 271, 13371.
      Quan, L.T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 1972.
      Teraoka, H., et al. 1996. FEBS Lett. 393, 1.
      Tiso, N., et al. 1996. Biochem. Biophys. Res. Commun. 225, 983.
      Xue, D., et al. 1996. Genes Dev. 10, 1073.
      Tewari, M., et al. 1995. Cell 81, 801.
      Fernandes-Alnemri, T., et al. 1994. J. Biol. Chem. 269, 30761.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
      Negative controltrpE (Ab-1)
      Positive controlRecombinant caspase-3 (Cat. No. 235417) or breast carcinoma tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Not Frozen Sections
      Not Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml)
      Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
      Frozen Sections (not recommended)
      Immunoprecipitation (not recommended)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant human caspase-3
      ImmunogenHuman
      CloneAM1.31-11
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AM65 0

      Documentation

      Anti-Caspase-3 (Ab-4) Mouse mAb (AM1.31-11) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Caspase-3 (Ab-4) Mouse mAb (AM1.31-11) Certificates of Analysis

      TitleLot Number
      AM65

      References

      Reference overview
      Alnemri, E.S. 1997. J. Cell. Biochem. 64, 33.
      Bayaert, R., et al. 1997. J. Biol. Chem. 272, 11694.
      Erhardt, P., et al. 1997. J. Biol. Chem. 272, 15049.
      Inayat-Hussain, S.H., et al. 1997 Hepatology 25, 1516.
      Krjewska, M., et al. 1997. Cancer Res. 57, 1605.
      Mashima, T., et al. 1997. Oncogene 14, 1007.
      McConnell, K.R., et al. 1997. J. Immunol. 158, 2083.
      Posmantur, R., et al. 1997. J. Neurochem. 68, 2328.
      Suzuki, A., et al. 1997. Exp. Cell. Res. 233, 48.
      Liu, X., et al. 1996. J. Biol. Chem. 271, 13371.
      Quan, L.T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 1972.
      Teraoka, H., et al. 1996. FEBS Lett. 393, 1.
      Tiso, N., et al. 1996. Biochem. Biophys. Res. Commun. 225, 983.
      Xue, D., et al. 1996. Genes Dev. 10, 1073.
      Tewari, M., et al. 1995. Cell 81, 801.
      Fernandes-Alnemri, T., et al. 1994. J. Biol. Chem. 269, 30761.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-August-2007 RFH
      SynonymsAnti-Apopain, Anti-CASP3, Anti-CPP32, Anti-ICE-3, Anti-LICE, Anti-YAMA
      ApplicationImmunoblotting (1 µg/ml)
      Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
      Frozen Sections (not recommended)
      Immunoprecipitation (not recommended)
      Application Data
      Detection of human caspase-3 by staining paraffin sections. Sample: Breast carcinoma tissue, paraffin-embedded. Primary antibody: Anti-Caspase-3 (Ab-4) Mouse mAb (AM1.31-11) (Cat. No. AM65) (2.5 µg/ml). Detection: DAB
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~11 kDa active subunit of caspase-3.
      BackgroundCaspase-3 (CPP32/Yama/Apopain) exists as a ~32 kDa inactive precursor protein in the cytoplasm of most cell types. Upon proteolytic activation the protein is cleaved at a specific site within the molecule to yield a heterodimer consisting of p20 and p11 subunits. The cleavage/activation proceeds in two steps yielding first a p3 fragment followed by the p20 and p11 fragments and is mediated by specific proteases including serine proteases and the enzyme granzyme B. Activation of Caspase-3 occurs rather rapidly following the onset of apoptosis induced by a number of different signals including but not restricted to TGFβ-1, TNF, and Fas. One of the most potent chemical activators of Caspase-3 is staurosporine. Once activated, Caspase-3 has a number of substrates itself. Among those which have been reported are MDM2, PITSLRE kinases, actin, DNA-PK, and PARP. Related to the C. elegans protein, Ced-3, Caspase-3 has been mapped in humans to chromosome 4q33-q35.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant human caspase-3
      CloneAM1.31-11
      IsotypeIgG2a
      Specieshuman, mouse
      Positive controlRecombinant caspase-3 (Cat. No. 235417) or breast carcinoma tissue
      Negative controltrpE (Ab-1)
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesAlnemri, E.S. 1997. J. Cell. Biochem. 64, 33.
      Bayaert, R., et al. 1997. J. Biol. Chem. 272, 11694.
      Erhardt, P., et al. 1997. J. Biol. Chem. 272, 15049.
      Inayat-Hussain, S.H., et al. 1997 Hepatology 25, 1516.
      Krjewska, M., et al. 1997. Cancer Res. 57, 1605.
      Mashima, T., et al. 1997. Oncogene 14, 1007.
      McConnell, K.R., et al. 1997. J. Immunol. 158, 2083.
      Posmantur, R., et al. 1997. J. Neurochem. 68, 2328.
      Suzuki, A., et al. 1997. Exp. Cell. Res. 233, 48.
      Liu, X., et al. 1996. J. Biol. Chem. 271, 13371.
      Quan, L.T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 1972.
      Teraoka, H., et al. 1996. FEBS Lett. 393, 1.
      Tiso, N., et al. 1996. Biochem. Biophys. Res. Commun. 225, 983.
      Xue, D., et al. 1996. Genes Dev. 10, 1073.
      Tewari, M., et al. 1995. Cell 81, 801.
      Fernandes-Alnemri, T., et al. 1994. J. Biol. Chem. 269, 30761.