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AP1165 Anti-FIS1 Mouse mAb (1G9)

Anti-FIS1, mouse monoclonal, clone 1G9, recognizes the ~15 kDa FIS1 protein in A431 cells and human liver and small intestine tissue. It is validated for use in ELISA, WB, and IHC (paraffin sections)

Anti-FIS1 Mouse mAb (1G9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TTC11, Anti-Fission 1 Homolog

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~15 kDa FIS1 protein in A431 cells and human liver and small intestine tissue.
      Catalogue NumberAP1165
      Brand Family Calbiochem®
      SynonymsAnti-TTC11, Anti-Fission 1 Homolog
      Application Data
      Detection of human FIS1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (1-5 µg/ml). Detection: chemiluninescence.

      Detection of human FIS1 by immunoblotting. Sample: Whole cell lysate from A-431 cells. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (1-5 µg/ml). Detection: chemiluninescence.

      Detection of human FIS1 by immunoblotting. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHuman liver tissue, A-431 cells, Human small intestine tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant human FIS1 (aa 1-153), expressed as a GST fusion protein
      Clone1G9
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1165 0

      Documentation

      Anti-FIS1 Mouse mAb (1G9) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-TTC11, Anti-Fission 1 Homolog
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human FIS1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (1-5 µg/ml). Detection: chemiluninescence.

      Detection of human FIS1 by immunoblotting. Sample: Whole cell lysate from A-431 cells. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (1-5 µg/ml). Detection: chemiluninescence.

      Detection of human FIS1 by immunoblotting. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~15 kDa FIS1 protein.
      BackgroundTTC11 promotes the fragmentation of the mitochondrial network and its perinuclear clustering. It can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. It interacts with DNM1L. The C terminus is required for mitochondrial localization, while the N teminus is necessary for mitochondrial fission.
      HostMouse
      ImmunogenFull-length, recombinant human FIS1 (aa 1-153), expressed as a GST fusion protein
      Clone1G9
      IsotypeIgG2b
      Specieshuman
      Positive controlHuman liver tissue, A-431 cells, Human small intestine tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review