Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AP1167 Anti-TNS4 Mouse mAb (3B8)

This Anti-TNS4 Mouse mAb (3B8) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of TNS4.

Anti-TNS4 Mouse mAb (3B8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CTEN, Anti-C Terminal Tensin Like Protein, Anti-Tensin 4

View Products on Sigmaaldrich.com
AP1167
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
AP1167-100UG 100 μg
Description
OverviewRecognizes the ~52 kDa TNS4 protein in HeLa cells.
Catalogue NumberAP1167
Brand Family Calbiochem®
SynonymsAnti-CTEN, Anti-C Terminal Tensin Like Protein, Anti-Tensin 4
Application Data
Detection of human TNS4 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with full-length, recombinant, human TNS4 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-TNS4 Mouse mAb (3B8) (Cat. No. AP1167) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TNS4 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TNS4 Mouse mAb (3B8) (Cat. No. AP1167) (10 µg/ml). Detection: fluorescence.
References
ReferencesSakashita, K., et al. 2008. Ann Surg Oncol. 15, 2606.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human TNS4 (aa 1-481), expressed as a GST fusion protein
Clone3B8
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1167-100UG 04055977212686

Documentation

Anti-TNS4 Mouse mAb (3B8) SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Sakashita, K., et al. 2008. Ann Surg Oncol. 15, 2606.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-October-2010 JLN
SynonymsAnti-CTEN, Anti-C Terminal Tensin Like Protein, Anti-Tensin 4
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human TNS4 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with full-length, recombinant, human TNS4 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-TNS4 Mouse mAb (3B8) (Cat. No. AP1167) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TNS4 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TNS4 Mouse mAb (3B8) (Cat. No. AP1167) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~52 kDa TNS4 protein.
BackgroundTNS4 may be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton. It may also promote apoptosis, via its cleavage by caspase 3.
HostMouse
ImmunogenFull-length, recombinant human TNS4 (aa 1-481), expressed as a GST fusion protein
Clone3B8
IsotypeIgG₁
Specieshuman
Positive controlHeLa cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesSakashita, K., et al. 2008. Ann Surg Oncol. 15, 2606.