05-1427 | Anti-ARF1 Antibody, clone 3F1

05-1427
100 µg  
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      Overview

      Replacement Information
      Replacement Information05-1427 is a recommended replacement for MAB3779

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ELISA, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1427
      ReplacesMAB3779
      DescriptionAnti-ARF1 Antibody, clone 3F1
      Alternate Names
      • ADP-ribosylation factor 1
      Background InformationARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide
      Applications
      ApplicationAnti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).
      Key Applications
      • Western Blotting
      • ELISA
      • Immunohistochemistry (Paraffin)
      Application NotesELISA: A previous lot of this antibody was used in ELISA.
      Optimal working dilutions must be determined by end user.

      Immunohistochemistry(paraffin): Representative testing from a previous lot.
      Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer
      Biological Information
      ImmunogenSynthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.
      EpitopeC-terminus
      Clone3F1
      HostMouse
      SpecificityRecognizes human ARF1
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • ARF1
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P84077 # GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
      SIZE: 181 amino acids; 20697 Da
      SUBUNIT: Interacts with PRKCABP, GGA1, DDEF2 and PIP5K1A (By similarity). Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Binds DDEF2. Interacts with ARHGAP21.
      SIMILARITY: SwissProt: P84077 ## Belongs to the small GTPase superfamily. Arf family
      Molecular Weight~21 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ARF1, clone 3F1 - NG1590610 NG1590610
      Anti-ARF1, clone 3F1 - NG1590617 NG1590617
      Anti-ARF1, clone 3F1, C-terminus - 2116005 2116005
      Anti-ARF1, clone 3F1, C-terminus - 2128248 2128248
      Anti-ARF1, clone 3F1, C-terminus - 2153064 2153064
      Anti-ARF1, clone 3F1, C-terminus - 2272153 2272153
      Anti-ARF1, clone 3F1, C-terminus - NG1726950 NG1726950
      Anti-ARF1, clone 3F1, C-terminus - NG1943280 NG1943280
      Anti-ARF1, clone 3F1, C-terminus -2557580 2557580
      Anti-ARF1, clone 3F1, C-terminus -2581303 2581303