06-465 Anti-Abl SH2 Domain Antibody

250 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, B, H, M, Po, RbIP, WBRbPurifiedPolyclonal Antibody
      Catalogue Number06-465
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Abl SH2 Domain Antibody
      OverviewThe amount of c-Abl tends to be low in many cell lines and c-Abl was not detected using RIPA lysates from human Jurkat, Raj, Hut lymphoid cell lines, or murine Wehi myeloid cell line
      Product Information
      Presentation0.1M Tris-glycine buffer, pH 7.4
      ApplicationDetect Abl SH2 Domain using this Anti-Abl SH2 Domain Antibody validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenRecombinant protein containing Glutathione S-transferase fused to the SH2 domain of Abl
      Specificityhuman p210bcr-abl, p140c-Abl, as well as viral p160gag-abl
      Species Reactivity
      • Avian
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11.
      Gene Symbol
      • ABL1
      • v-abl
      • JTK7
      • p150
      • c-ABL
      • bcr/abl
      • ABL
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00519 # Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.
      COFACTOR: Magnesium or manganese.
      SIZE: 1130 amino acids; 122873 Da
      SUBUNIT: Interacts with SORBS1 following insulin stimulation. Found in a trimolecular complex containing CDK5 and CABLES1. Interacts with CABLES1 and PSTPIP1. Interacts with ZDHHC16 (By similarity). Interacts with INPPL1/SHIP2.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=The myristoylated c-ABL protein is reported to be nuclear.
      TISSUE SPECIFICITY: Widely expressed.
      PTM: Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation. Isoform IB is myristoylated on Gly-2.
      DISEASE: SwissProt: P00519 # A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
      SIMILARITY: SwissProt: P00519 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight140kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on human K562 leukemia cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-Abl SH2 Domain - 1433514335
      Anti-Mouse Abl SH2 domain - DAM1579943DAM1579943


      Reference overviewApplicationPub Med ID
      A potential SH3 domain-binding site in the Crk SH2 domain
      Anafi, M., et al
      J Biol Chem, 271:21365-74 (1996)  1996

      Kinase Assay8702917 8702917
      Abl tyrosine protein kinase.
      Laneuville, P
      Semin. Immunol., 7: 255-66 (1995)  1995

      Show Abstract
      8520030 8520030
      Abl-mediated transformation, immunoglobulin gene rearrangements and arrest of B lymphocyte differentiation.
      Rosenberg, N
      Semin. Cancer Biol., 5: 95-102 (1994)  1994

      Show Abstract
      8061334 8061334
      T-cell immunity to oncogenic proteins including mutated ras and chimeric bcr-abl.
      Cheever, M A, et al.
      Ann. N. Y. Acad. Sci., 690: 101-12 (1993)  1993

      Show Abstract
      8103658 8103658
      Abl tyrosine kinase in signal transduction and cell-cycle regulation.
      Wang, J Y
      Curr. Opin. Genet. Dev., 3: 35-43 (1993)  1993

      Show Abstract
      8453272 8453272

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      Life Science Research > Antibodies and Assays > Primary Antibodies