05-384 | Anti-α-Actinin Antibody, clone AT6/172

05-384
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RbICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-384
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-α-Actinin Antibody, clone AT6/172
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
      PresentationPlease see product datasheet for lot-specific formulation.
      Applications
      ApplicationThis Anti-α-Actinin Antibody, clone AT6/172 is validated for use in IC, WB for the detection of α-Actinin.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunoprecipitation is not recommended
      Biological Information
      ImmunogenHuman α-actinin
      CloneAT6/172
      HostMouse
      SpecificityRecognizes α-actinin.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.
      Gene Symbol
      • ACTN1
      • FLJ40884
      • Alpha-actinin-1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12814 # F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
      SIZE: 892 amino acids; 103058 Da
      SUBUNIT: Homodimer; antiparallel. Interacts with MYOZ2, PDLIM2 and TTID and LPP.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z-disk. Note=Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
      SIMILARITY: SwissProt: P12814 ## Belongs to the alpha-actinin family. & Contains 1 actin-binding domain. & Contains 2 CH (calponin-homology) domains. & Contains 2 EF-hand domains. & Contains 4 spectrin repeats.
      Molecular Weight100 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in immunoblot on human A431 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-#945;-Actinin, clone AT6/172 (mouse monoclonal IgG1) - 2144396 2144396
      Anti-#945;-Actinin, clone AT6/172 - 2029690 2029690
      Anti--Actinin, clone AT6/172 - DAM1567767 DAM1567767
      Anti-a-Actinin, clone AT6/172 (mouse monoclonal IgG1) 2901513
      Anti-a-Actinin, clone AT6/172 (mouse monoclonal IgG1) - 2198984 2198984
      Anti-a-Actinin, clone AT6/172 (mouse monoclonal IgG1) - 2207266 2207266
      Anti-a-Actinin, clone AT6/172 (mouse monoclonal IgG1) - 2273966 2273966
      Anti-a-Actinin, clone AT6/172 (mouse monoclonal IgG1) - 2297217 2297217
      Anti-a-Actinin, clone AT6/172 - DAM1416544 DAM1416544
      Anti-a-Actinin, clone AT6/172 - DAM1503376 DAM1503376

      References

      Reference overviewApplicationPub Med ID
      Mining the human complexome database identifies RBM14 as an XPO1-associated protein involved in HIV-1 Rev function.
      Budhiraja, S; Liu, H; Couturier, J; Malovannaya, A; Qin, J; Lewis, DE; Rice, AP
      Journal of virology  89  3557-67  2015

      Show Abstract
      25589658 25589658
      Never in mitosis gene A related kinase-6 attenuates pressure overload-induced activation of the protein kinase B pathway and cardiac hypertrophy.
      Bian, Z; Liao, H; Zhang, Y; Wu, Q; Zhou, H; Yang, Z; Fu, J; Wang, T; Yan, L; Shen, D; Li, H; Tang, Q
      PloS one  9  e96095  2014

      Show Abstract
      24763737 24763737
      Fibroblast growth factor receptors as novel therapeutic targets in SNF5-deleted malignant rhabdoid tumors.
      Wöhrle, S; Weiss, A; Ito, M; Kauffmann, A; Murakami, M; Jagani, Z; Thuery, A; Bauer-Probst, B; Reimann, F; Stamm, C; Pornon, A; Romanet, V; Guagnano, V; Brümmendorf, T; Sellers, WR; Hofmann, F; Roberts, CW; Graus Porta, D
      PloS one  8  e77652  2013

      Show Abstract
      24204904 24204904
      IKKi deficiency promotes pressure overload-induced cardiac hypertrophy and fibrosis.
      Dai, J; Shen, DF; Bian, ZY; Zhou, H; Gan, HW; Zong, J; Deng, W; Yuan, Y; Li, F; Wu, QQ; Gao, L; Zhang, R; Ma, ZG; Li, HL; Tang, QZ
      PloS one  8  e53412  2013

      Show Abstract
      Immunofluorescence23349709 23349709
      Proprotein convertases play an important role in regulating PKGI endoproteolytic cleavage and nuclear transport.
      Kato, S; Zhang, R; Roberts, JD
      American journal of physiology. Lung cellular and molecular physiology  305  L130-40  2013

      Show Abstract
      23686857 23686857
      Regulation of focal adhesions by flightless i involves inhibition of paxillin phosphorylation via a Rac1-dependent pathway.
      Kopecki, Z; O'Neill, GM; Arkell, RM; Cowin, AJ
      The Journal of investigative dermatology  131  1450-9  2011

      Show Abstract
      21430700 21430700
      Activating transcription factor 3 deficiency promotes cardiac hypertrophy, dysfunction, and fibrosis induced by pressure overload.
      Zhou, H; Shen, DF; Bian, ZY; Zong, J; Deng, W; Zhang, Y; Guo, YY; Li, H; Tang, QZ
      PloS one  6  e26744  2011

      Show Abstract Full Text Article
      22053207 22053207
      Flightless I regulates hemidesmosome formation and integrin-mediated cellular adhesion and migration during wound repair.
      Kopecki, Z; Arkell, R; Powell, BC; Cowin, AJ
      The Journal of investigative dermatology  129  2031-45  2009

      Show Abstract
      19212345 19212345
      Dynamic interplay of transcriptional machinery and chromatin regulates "late" expression of the chemokine RANTES in T lymphocytes.
      Ahn, YT; Huang, B; McPherson, L; Clayberger, C; Krensky, AM
      Molecular and cellular biology  27  253-66  2007

      Show Abstract Full Text Article
      17074812 17074812
      Identification of genes differentially expressed in mouse mammary epithelium transformed by an activated beta-catenin.
      Renou, JP; Bierie, B; Miyoshi, K; Cui, Y; Djiane, J; Reichenstein, M; Shani, M; Hennighausen, L
      Oncogene  22  4594-610  2003

      Show Abstract
      12881717 12881717

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies