05-665-AF647 | Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 647

05-665-AF647
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, FCMAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue Number05-665-AF647
      DescriptionAnti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 647
      Alternate Names
      • Catenin beta-1
      • Beta-catenin
      • Active-β-Catenin
      Background InformationCatenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a “destruction complex” with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
      Applications
      ApplicationThis Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 647 is validated for use in Immunocytochemistry, Flow Cytometry for the detection of Active-β-Catenin.
      Key Applications
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Active-β-Catenin in NIH/3T3 cells.
      Flow Cytometry Analysis: 0.1 µg of this antibody from a representative lot detected Active-β-Catenin in 1X10E6 A431 and HeLa cells.
      The unconjugated version (Cat. No. 05-665) has been shown to work in WB, FC, ICC, IHC, IH(P).
      Biological Information
      ImmunogenFusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.
      EpitopeThe epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.
      Clone8E7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityRecognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Zebrafish based on 100% sequence homology. The antibody eptiope is 100% conserved in zebrafish.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CTNNB1
      • CTNNB
      • PRO2286
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~85 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Active-β-Catenin in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 647 2975211
      Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 647 -2892862 2892862
      Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 647 -Q2592808 Q2592808

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies