04-085 Anti-Ago family Antibody

100 assays  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Catalogue Number04-085
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Ago family Antibody
      Alternate Names
      • argonaute family
      • piwi
      Product Information
      Presentation100 μg of Protein A purified mouse monoclonal IgG1κ in 100μl of 1X PBS, pH 7.0, 0.1% Azide.
      ApplicationUse Anti-Ago family Antibody (Mouse Monoclonal Antibody) validated in WB to detect Ago family also known as argonaute family PIWI.
      Key Applications
      • Western Blotting
      Application NotesSuggested dilution:
      Western Blot: 1:500
      Biological Information
      ImmunogenSynthetic peptide PKIDVYHYEVDIKPDKCPRRVNREVV
      SpecificityAgo family proteins
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4.
      Gene Symbol
      • EIF2C1
      • AGO1
      • EIF2C
      • Argonaute-1
      • GERP95
      • Q99
      • DKFZp686M13167
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UL18 # Plays an important role in the eukaryotic peptide chain initiation process.
      SIZE: 857 amino acids; 97214 Da
      SIMILARITY: SwissProt: Q9UL18 ## Belongs to the argonaute family. & Contains 1 PAZ domain. & Contains 1 Piwi domain.
      Molecular Weight~100 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ago family (Mouse monoclonal IgG1κ)2969555
      Anti-Ago family (Mouse monoclonal IgG1κ)2981056
      Anti-Ago family - 07010493130701049313
      Anti-Ago family - DAM1394925DAM1394925
      Anti-Ago family -25715962571596
      Anti-Ago family -26405332640533
      Anti-Ago family -27415192741519


      Reference overviewPub Med ID
      Differential remodeling of mono- and trimethylated H3K27 during porcine embryo development.
      Park KE, Magnani L, Cabot RA
      Mol Reprod Dev  76  1033-42.  2009

      Show Abstract
      19536841 19536841
      Overlapping functions of argonaute proteins in patterning and morphogenesis of Drosophila embryos
      Meyer, Wibke J, et al
      PLoS Genet, 2:e134 (2006)  2006

      16934003 16934003
      Interactions between the RNA interference effector protein Ago1 and 14-3-3 proteins: consequences for cell cycle progression
      Stoica, Cezar, et al
      J Biol Chem, 281:37646-51 (2006)  2006

      17043360 17043360
      Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi
      Yigit, Erbay, et al
      Cell, 127:747-57 (2006)  2006

      17110334 17110334


      RNA-Binding Protein Immunoprecipitation