05-796 | Anti-Akt1/PKBα Antibody, clone AW24, rabbit monoclonal

05-796
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-796
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Akt1/PKBα Antibody, clone AW24, rabbit monoclonal
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect Akt1/PKBα using this Anti-Akt1 Antibody, clone AW24 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenPeptide (C-RPHFPQFSYSASGTA) corresponding to the C-terminal (residues 466-480) of rat Akt1/PKBα
      Cloneclone AW24
      HostRabbit
      SpecificityAkt1/PKBα
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • RAC-ALPHA
      • PKB
      • MGC99656
      • RAC-PK-alpha
      • C-AKT
      • AKT
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight~60kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from human HEK293 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Akt1/PKB#945;, clone AW24 - DAM1813885 DAM1813885
      Anti-Akt1/PKBa, clone AW24 (rabbit monoclonal IgG) 2890798
      Anti-Akt1/PKBa, clone AW24 (rabbit monoclonal IgG) - 2194159 2194159
      Anti-Akt1/PKBa, clone AW24 - JBC1839909 JBC1839909
      Anti-Akt1/PKBa, clone AW24 - JBC1841959 JBC1841959
      Anti-Akt1/PKBalpha;, clone AW24 - 26860 26860
      Anti-Akt1/PKBα, clone AW24 (rabbit monoclonal IgG) - 2382157 2382157
      Anti-Akt1/PKBα, clone AW24 -2658766 2658766
      Anti-Akt1/PKBα, clone AW24 -2679363 2679363
      Anti-Akt1/PKBα, clone AW24 -2717630 2717630

      References

      Reference overviewPub Med ID
      Temsirolimus activates autophagy and ameliorates cardiomyopathy caused by lamin A/C gene mutation.
      Choi, JC; Muchir, A; Wu, W; Iwata, S; Homma, S; Morrow, JP; Worman, HJ
      Science translational medicine  4  144ra102  2012

      Show Abstract
      22837537 22837537
      SILAM for quantitative proteomics of liver Akt1/PKBα after burn injury.
      Lu, XM; Tompkins, RG; Fischman, AJ
      International journal of molecular medicine  29  461-71  2012

      Show Abstract
      22179310 22179310
      UPR-mediated TRIB3 expression correlates with reduced AKT phosphorylation and inability of interleukin 6 to overcome palmitate-induced apoptosis in RINm5F cells.
      Nicoletti-Carvalho, JE; Nogueira, TC; Gorjão, R; Bromati, CR; Yamanaka, TS; Boschero, AC; Velloso, LA; Curi, R; Anhê, GF; Bordin, S
      The Journal of endocrinology  206  183-93  2010

      Show Abstract
      20488947 20488947
      Specific binding of the Akt-1 protein kinase to phosphatidylinositol 3,4,5-trisphosphate without subsequent activation.
      James, SR; Downes, CP; Gigg, R; Grove, SJ; Holmes, AB; Alessi, DR
      The Biochemical journal  315 ( Pt 3)  709-13  1996

      Show Abstract
      8645147 8645147

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      Western Blotting Tools

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      Life Science Research > Antibodies and Assays > Primary Antibodies