06-680 | Anti-Androgen Receptor Antibody

06-680
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IP, FC, ChIP, IHC, ChIP-seqRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-680
      DescriptionAnti-Androgen Receptor Antibody
      Alternate Names
      • Androgen receptor
      • Dihydrotestosterone receptor
      • Nuclear receptor subfamily 3 group C member 4
      Background InformationThe androgen receptor (AR) is a type of nuclear receptor which is activated by binding of either of the androgenic hormones testosterone or dihydrotestosterone. The main function of the androgen receptor is as a DNA binding transcription factor which regulates gene expression. However the androgen receptor also has additional functions independent of DNA binding. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Androgen Receptor Antibody, PG-21 is a Rabbit Polyclonal Antibody for detection of Androgen Receptor also known as Dihydrotestosterone Receptor & has been tested in ICC, IHC, IP & WB.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Flow Cytometry
      • Chromatin Immunoprecipitation (ChIP)
      • Immunohistochemistry
      • ChIP-seq
      Application NotesImmunoprecipitation Analysis: 34 µL from a representative lot immunoprecipitated Androgen Receptor from 1000 µg of LNCaP cell lysate.

      Flow Cytometry Analysis: A 1:300 dilution from a representative lot detected Androgen Receptor in 0.2X10E6 MCF-7 cells.

      Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Li, X., et al. (2011). PLoS One. 6(9):e25040.).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in embryonic rat tissue, neonatal rat testis tissue, and adult rat testis tissue (Nation, T., et al. (2010). J Pediatr Surg. 46(8): 1539-1543.).

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in MCF-7 cells stabily transfected with Androgen Receptor (Ghatge, R. P., et al. (2005). Breast Cancer Res. 7(6):R1036-1050.).

      Chromatin Immunoprecipitaton-Sequence Analysis: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from LNCaP cell lysates under certain treated conditions (Zhao, J. C., et al. (2012). Genome Res. 22(2):322-331.).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of rat Androgen Receptor.
      EpitopeThis antibody recognizes the Modulation Region within the N-terminus of Androgen Receptor.
      HostRabbit
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with Human and Rat. Predicted to react with Mouse, Bovine, Horse, and Canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ar
      • Nr3c4
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~110 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in LNCaP cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Androgen Receptor in 10 µg of LNCaP cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Androgen Receptor 2462877
      Anti-Androgen Receptor - 2387519 2387519
      Anti-Androgen Receptor - 2328016 2328016
      Anti-Androgen Receptor - 2489005 2489005
      Anti-Androgen Receptor - 2500466 2500466
      Anti-Androgen Receptor -2551210 2551210
      Anti-Androgen Receptor -2585789 2585789
      Anti-Androgen Receptor -2605374 2605374
      Anti-Androgen Receptor -2638891 2638891
      Anti-Androgen Receptor -2639117 2639117

      References

      Reference overviewApplicationSpeciesPub Med ID
      Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture.
      Reddy, RC; Amodei, R; Estill, CT; Stormshak, F; Meaker, M; Roselli, CE
      PloS one  10  e0129521  2015

      Show Abstract
      26053052 26053052
      Divergent androgen regulation of unfolded protein response pathways drives prostate cancer.
      Sheng, X; Arnoldussen, YJ; Storm, M; Tesikova, M; Nenseth, HZ; Zhao, S; Fazli, L; Rennie, P; Risberg, B; Wæhre, H; Danielsen, H; Mills, IG; Jin, Y; Hotamisligil, G; Saatcioglu, F
      EMBO molecular medicine  7  788-801  2015

      Show Abstract
      25864123 25864123
      Pten Regulates Epithelial Cytodifferentiation during Prostate Development.
      Lokody, IB; Francis, JC; Gardiner, JR; Erler, JT; Swain, A
      PloS one  10  e0129470  2015

      Show Abstract
      26076167 26076167
      Androgen Receptor-Mediated Growth Suppression of HPr-1AR and PC3-Lenti-AR Prostate Epithelial Cells.
      Kim, YC; Chen, C; Bolton, EC
      PloS one  10  e0138286  2015

      Show Abstract
      26372468 26372468
      Therapeutic targeting of BET bromodomain proteins in castration-resistant prostate cancer.
      Asangani, IA; Dommeti, VL; Wang, X; Malik, R; Cieslik, M; Yang, R; Escara-Wilke, J; Wilder-Romans, K; Dhanireddy, S; Engelke, C; Iyer, MK; Jing, X; Wu, YM; Cao, X; Qin, ZS; Wang, S; Feng, FY; Chinnaiyan, AM
      Nature  510  278-82  2014

      Show Abstract
      24759320 24759320
      Characterization of aromatase expression in the adult male and female mouse brain. I. Coexistence with oestrogen receptors α and β, and androgen receptors.
      Stanić, D; Dubois, S; Chua, HK; Tonge, B; Rinehart, N; Horne, MK; Boon, WC
      PloS one  9  e90451  2014

      Show Abstract
      24646567 24646567
      Cooperativity and equilibrium with FOXA1 define the androgen receptor transcriptional program.
      Jin, HJ; Zhao, JC; Wu, L; Kim, J; Yu, J
      Nature communications  5  3972  2014

      Show Abstract
      Western Blotting24875621 24875621
      Autophagy pathway is required for IL-6 induced neuroendocrine differentiation and chemoresistance of prostate cancer LNCaP cells.
      Chang, PC; Wang, TY; Chang, YT; Chu, CY; Lee, CL; Hsu, HW; Zhou, TA; Wu, Z; Kim, RH; Desai, SJ; Liu, S; Kung, HJ
      PloS one  9  e88556  2014

      Show Abstract
      Western BlottingHuman24551118 24551118
      Negative regulation of the androgen receptor gene through a primate-specific androgen response element present in the 5' UTR.
      Hay, CW; Watt, K; Hunter, I; Lavery, DN; MacKenzie, A; McEwan, IJ
      Hormones & cancer  5  299-311  2014

      Show Abstract
      24895212 24895212
      A hormone-dependent feedback-loop controls androgen receptor levels by limiting MID1, a novel translation enhancer and promoter of oncogenic signaling.
      Köhler, A; Demir, U; Kickstein, E; Krauss, S; Aigner, J; Aranda-Orgillés, B; Karagiannidis, AI; Achmüller, C; Bu, H; Wunderlich, A; Schweiger, MR; Schaefer, G; Schweiger, S; Klocker, H; Schneider, R
      Molecular cancer  13  146  2014

      Show Abstract
      24913494 24913494

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      06-680-AF488 Anti-Androgen Receptor Antibody, Alexa Fluor® 488 Conjugate Show Pricing & Availability
      06-680-AF647 Anti-Androgen Receptor Antibody, Alexa Fluor® 647 Conjugate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies