05-1579 | Anti-Axin-1 Antibody, clone A5

05-1579
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1579
      DescriptionAnti-Axin-1 Antibody, clone A5
      Alternate Names
      • Axis inhibition protein 1
      • axin 1
      • axis inhibitor 1
      • fused, mouse, homolog of
      Background InformationAxin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.
      References
      Product Information
      FormatPurified
      Control
      • SW-480 cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationDetect Axin-1 using this Anti-Axin-1 Antibody, clone A5 validated for use in WB, IP & IC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1.
      EpitopeN-terminus
      CloneA5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the N-terminus of Axin-1.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AXIN
      • hAxin
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of beta-catenin and APC by GSK3B. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 By similarity. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation.

      SUBUNIT STRUCTURE: Homodimer. Interacts with GSK3B and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Ternary complex. Also binds to plakoglobin (gamma-catenin), APC, DVL and PP2A. Interacts with SMAD6, SMAD7 and RNF111 By similarity. Interacts with TP53 and HIPK2. Probably part of a complex consisting of TP53, HIPK2 and AXIN1. Interacts with DIXDC1; prevents interaction with MAP3K1. Interacts with MAP3K4, MDFI and MDFIC. Interacts with ANKRD6. Interacts with AIDA.

      SUBCELLULAR LOCATION: Cytoplasm By similarity.

      TISSUE SPECIFICITY: Expressed in embryonic stem cells.

      DEVELOPMENTAL STAGE: Widely expressed at E10.5 to E16.5 day.

      PTM: Probably phosphorylated by GSK3B and dephosphorylated by PP2A.

      SEQUENCE SIMILARITIES: Contains 1 DIX domain.

      Contains 1 RGS domain.
      Molecular Weight~ 97 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Axin-1, clone A5 - 2142200 2142200
      Anti-Axin-1, clone A5 - 2031363 2031363
      Anti-Axin-1, clone A5 - 2107260 2107260
      Anti-Axin-1, clone A5 - 2216909 2216909
      Anti-Axin-1, clone A5 - 2565743 2565743
      Anti-Axin-1, clone A5 - NG1842851 NG1842851
      Anti-Axin-1, clone A5 - NG1901590 NG1901590
      Anti-Axin-1, clone A5 - NRG1702348 NRG1702348
      Anti-Axin-1, clone A5 -2524627 2524627
      Anti-Axin-1, clone A5 -2615680 2615680

      References

      Reference overviewApplicationPub Med ID
      MITF drives endolysosomal biogenesis and potentiates Wnt signaling in melanoma cells.
      Ploper, D; Taelman, VF; Robert, L; Perez, BS; Titz, B; Chen, HW; Graeber, TG; von Euw, E; Ribas, A; De Robertis, EM
      Proceedings of the National Academy of Sciences of the United States of America  112  E420-9  2015

      Show Abstract
      Immunofluorescence25605940 25605940
      Phosphatidylinositol 3-kinase signaling does not activate the wnt cascade.
      Ng, SS; Mahmoudi, T; Danenberg, E; Bejaoui, I; de Lau, W; Korswagen, HC; Schutte, M; Clevers, H
      The Journal of biological chemistry  284  35308-13  2009

      Show Abstract
      19850932 19850932

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies