05-729 | Anti-Bcl2 Antibody, clone 100

05-729
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ca, FeFC, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-729
      Replaces04-436
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Bcl2 Antibody, clone 100
      Alternate Names
      • B-cell CLL/lymphoma 2
      • B-cell lymphoma protein 2
      Background InformationThe Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Apoptosis/survival is regulated by the relative balance of pro-apoptotic (Bax, BclXS, Bak, Bad) and anti-apoptotic (Bcl2, BclXL, Mcl-1, Dad-1) family members, and their heterodimerization. Bcl2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders.
      References
      Product Information
      FormatPurified
      Control
      • Tonsil tissue.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 70% storage buffer PBS with 0.1% sodium azide and 30% glycerol. Store at -20ºC.
      Applications
      ApplicationDetect Bcl2 using this Anti-Bcl2 Antibody, clone 100 validated for use in FC, IH & WB.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      An independent laboratory has reported that this antibody detects Bcl2 in paraffin and acetone-fixed tissues.

      FACS Analysis:
      Recommended
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 41-54 of human Bcl2 protein. The immunizing sequence is identical in canine and feline.
      Epitopea.a. 41-54
      Clone100
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Bcl2, Mr 26 kDa.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Canine
      • Feline
      Species Reactivity NoteHuman. Predicted to cross-react with feline and canine, based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
      Gene Symbol
      • BCL2
      • Bcl-2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
      SIZE: 239 amino acids; 26266 Da
      SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.
      SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
      TISSUE SPECIFICITY: Expressed in a variety of tissues.
      DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
      PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
      DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
      SIMILARITY: Belongs to the Bcl-2 family.
      Molecular Weight26 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on Raji RIPA cell lysate.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected Bcl2 in a Raji RIPA cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Bcl2, clone 100 - 2152221 2152221
      Anti-Bcl2, clone 100 - 1958103 1958103
      Anti-Bcl2, clone 100 - 1984836 1984836
      Anti-Bcl2, clone 100 - 2037621 2037621
      Anti-Bcl2, clone 100 - 2262774 2262774
      Anti-Bcl2, clone 100 - 2495653 2495653
      Anti-Bcl2, clone 100 - 26504 26504
      Anti-Bcl2, clone 100 - 27804 27804
      Anti-Bcl2, clone 100 - 31706 31706
      Anti-Bcl2, clone 100 - 33553 33553

      References

      Reference overviewApplicationSpeciesPub Med ID
      Combination of 12-O-tetradecanoylphorbol-13-acetate with diethyldithiocarbamate markedly inhibits pancreatic cancer cell growth in 3D culture and in immunodeficient mice.
      Huang, H; Cao, K; Malik, S; Zhang, Q; Li, D; Chang, R; Wang, H; Lin, W; Van Doren, J; Zhang, K; Du, Z; Zheng, X
      International journal of molecular medicine  35  1617-24  2015

      Show Abstract
      25847449 25847449
      Aβ1-42 monomers or oligomers have different effects on autophagy and apoptosis.
      Guglielmotto, M; Monteleone, D; Piras, A; Valsecchi, V; Tropiano, M; Ariano, S; Fornaro, M; Vercelli, A; Puyal, J; Arancio, O; Tabaton, M; Tamagno, E
      Autophagy  10  1827-43  2014

      Show Abstract
      25136804 25136804
      MART-10, a New Generation of Vitamin D Analog, Is More Potent than 1α,25-Dihydroxyvitamin D(3) in Inhibiting Cell Proliferation and Inducing Apoptosis in ER+ MCF-7 Breast Cancer Cells.
      Chiang, KC; Yeh, CN; Chen, SC; Shen, SC; Hsu, JT; Yeh, TS; Pang, JH; Su, LJ; Takano, M; Kittaka, A; Juang, HH; Chen, TC
      Evidence-based complementary and alternative medicine : eCAM  2012  310872  2012

      Show Abstract
      23304196 23304196
      Glyoxalase-I is a novel target against Bcr-Abl+ leukemic cells acquiring stem-like characteristics in a hypoxic environment.
      Takeuchi, M; Kimura, S; Kuroda, J; Ashihara, E; Kawatani, M; Osada, H; Umezawa, K; Yasui, E; Imoto, M; Tsuruo, T; Yokota, A; Tanaka, R; Nagao, R; Nakahata, T; Fujiyama, Y; Maekawa, T
      Cell death and differentiation  17  1211-20  2010

      Show Abstract
      Western Blotting20139893 20139893
      Suppressor of cytokine signaling 3 sensitizes anaplastic thyroid cancer to standard chemotherapy.
      Francipane, MG; Eterno, V; Spina, V; Bini, M; Scerrino, G; Buscemi, G; Gulotta, G; Todaro, M; Dieli, F; De Maria, R; Stassi, G
      Cancer research  69  6141-8  2009

      Show Abstract
      Western BlottingHuman19638576 19638576
      Involvement of mitochondrial permeability transition pore opening in alpha-bisabolol induced apoptosis.
      Cavalieri, Elisabetta, et al.
      FEBS J., 276: 3990-4000 (2009)  2009

      Show Abstract
      19570051 19570051
      VIP and VIP gene silencing modulation of differentiation marker N-cadherin and cell shape of corneal endothelium in human corneas ex vivo.
      Koh, SW; Chandrasekara, K; Abbondandolo, CJ; Coll, TJ; Rutzen, AR
      Investigative ophthalmology & visual science  49  3491-8  2008

      Show Abstract
      18441300 18441300
      Human lactoferrin exerts bi-directional actions on PC12 cell survival via ERK1/2 pathway.
      Lin, Tsung-Yao, et al.
      Biochem. Biophys. Res. Commun., 337: 330-6 (2005)  2005

      Show Abstract
      16188236 16188236
      bcl-2 protein in non-small-cell lung carcinoma.
      Pezzella, F, et al.
      N. Engl. J. Med., 329: 690-4 (1993)  1993

      Show Abstract
      8393963 8393963
      Evaluation of bcl-2 protein expression and 14;18 translocation as prognostic markers in follicular lymphoma.
      Pezzella, F, et al.
      Br. J. Cancer, 65: 87-9 (1992)  1992

      Show Abstract
      1733447 1733447

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies