05-637-25UG | Anti-Bmi-1 Antibody, clone F6 (mouse monoclonal IgG1)

25 μg  
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      Replacement Information
      Catalogue Number05-637-25UG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Bmi-1 Antibody, clone F6 (mouse monoclonal IgG1)
      Alternate Names
      • Polycomb group RING finger protein 4
      • RING finger protein 51
      Background InformationThe Bmi1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors.
      Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Indeed, based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.
      Product Information
      • U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos
      PresentationImmunoaffinity Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
      ApplicationAnti-Bmi-1 Antibody, clone F6 is a Mouse Monoclonal Antibody for detection of Bmi-1 also known as Polycomb group RING finger protein 4, RING finger protein 51 and has been validated in ICC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenImmunized with recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityRecognizes Bmi-1 (triplet).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BMI1
      • MGC12685
      • PCGF4
      • flvi-2/bmi-1
      • RNF51
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35226 # Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
      SIZE: 326 amino acids; 36949 Da
      SUBUNIT: Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1- like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP.
      PTM: May be polyubiquitinated; which does not lead to proteasomal degradation.DISEASE:SwissProt: P35226 # Cooperates with the MYC oncogene to produce B-lymphomas.
      SIMILARITY: SwissProt: P35226 ## Contains 1 RING-type zinc finger.
      Molecular Weight40-44 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from U2OS cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information


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