05-633 | Anti-BrdU Antibody, clone BU-1

05-633
4 vials  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AICCMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number05-633
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-BrdU Antibody, clone BU-1
      Background InformationThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
      References
      Product Information
      FormatCulture Supernatant
      Presentation4 vials, each vial containing mouse culture supernatant lyophilized from 2.5 mL. The supernatant contains mycoplasma, which contribute enzymes that facilitate antibody binding. Lyophilized powder.
      Applications
      ApplicationDetect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC.
      Key Applications
      • Immunocytochemistry
      Biological Information
      Immunogen5-iodouridine:ovalbumin conjugate
      Cloneclone BU-1
      HostMouse
      Specificity5-Bromo-2-deoxyuridine
      IsotypeIgG2a
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodConcentrated culture supernatant
      Molecular Weightnone given
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceA representative lot was evaluated by immunocytochemistry on HeLa cells. A 1:2-1:4 dilution from a representative lot showed positive immunostaining for Anti-BrdU in HeLa cells fixed with 95% ethanol, 5% glacial acetic acid. This lot was tested using the DNA Replication Assay Kit (Catalog # 17-316).

      Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size4 vials
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-BrdU, clone BU-1 (mouse culture supernatant, IgG2a) - DAM1472193 DAM1472193
      Anti-BrdU, clone BU-1 - 2373343 2373343
      Anti-BrdU, clone BU-1 - 1959681 1959681
      Anti-BrdU, clone BU-1 - 2020539 2020539
      Anti-BrdU, clone BU-1 - 2056625 2056625
      Anti-BrdU, clone BU-1 - 2064511 2064511
      Anti-BrdU, clone BU-1 - 22079 22079
      Anti-BrdU, clone BU-1 - 25095 25095
      Anti-BrdU, clone BU-1 - 33345 33345
      Anti-BrdU, clone BU-1 - DAM1576922 DAM1576922

      References

      Reference overviewPub Med ID
      Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
      Tudor, CS; Bruns, H; Daniel, C; Distel, LV; Hartmann, A; Gerbitz, A; Buettner, MJ
      PloS one  9  e114345  2014

      Show Abstract
      25470820 25470820
      Difference between random and imprinted X inactivation in common voles.
      Elena V Dementyeva,Alexander I Shevchenko,Olga V Anopriyenko,Nina A Mazurok,Eugeny A Elisaphenko,Tatyana B Nesterova,Neil Brockdorff,Suren M Zakian
      Chromosoma  119  2010

      Show Abstract
      20473512 20473512
      Models of S-phase determination in lymphomas from flow cytometric DNA content histograms: comparison with the bromodeoxyuridine labeling index.
      Beauregard, P, et al.
      Hematologic pathology, 5: 177-83 (1991)  1991

      1794967 1794967
      Immunofluorescence labeling indices in myeloma and related monoclonal gammopathies.
      Greipp, P R, et al.
      Mayo Clin. Proc., 62: 969-77 (1987)  1987

      Show Abstract
      3118117 3118117
      S-phase detection with an antibody to bromodeoxyuridine. Role of DNase pretreatment.
      Gonchoroff, N J, et al.
      J. Immunol. Methods, 93: 97-101 (1986)  1986

      Show Abstract
      3772110 3772110
      A monoclonal antibody reactive with 5-bromo-2-deoxyuridine that does not require DNA denaturation.
      Gonchoroff, N J, et al.
      Cytometry, 6: 506-12 (1985)  1985

      Show Abstract
      3905299 3905299

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      Life Science Research > Antibodies and Assays > Primary Antibodies