06-1061 | Anti-CARD11 Antibody

06-1061
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Eq, CaWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1061
      DescriptionAnti-CARD11 Antibody
      Alternate Names
      • caspase recruitment domain family, member 11
      • card-maguk protein 1
      • Carma 1
      • bcl10-interacting maguk protein 3
      • CARD-containing MAGUK protein 3
      Background InformationCARD11 is part of a group of CARD containing proteins, or proteins that contain caspase recruitment domain, belonging to the membrane-associated guanylate kinase (MAGUK) family functioning as molecular scaffolds at specialized locations of the plasma membrane for the production of multiprotein complexes. The CARD domain is a module for protein-binding that helps to regulate the production of CARD-containing proteins into complexes that signal NF-κB. CARD11 is a multidomain adaptor that is necessary for T-cell receptor (TCR) signaling activation of NF-κB as a necessary component of T-cell activation during adaptive immune response. In this pathway, CARD11 undergoes phosphorylation between its PDZ and coiled-coil domains by protein kinase D (PKC) on serine residues 564, 577, and 657 in order to activate NF-κB. Along with immunity, NF-κB is a critical component in the gene activation for pathways such as inflammation and apoptosis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Daudi cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-CARD11 Antibody detects level of CARD11 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot (SNAP ID) Analysis: 0.25 µg/mL of this antibody detected CARD11 in 10 µg of Daudi cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the internal domain of human CARD11.
      EpitopeInternal Domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the internal domain of CARD11.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Horse
      • Canine
      Species Reactivity NoteDemonstrated to react with human.
      Predicted to react with mouse, rat, equine, and canine based on 100% sequence homology.
      Bovine (95% sequence homology). Chicken (86% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq].
      Gene Symbol
      • CARMA1
      • CARD11
      • BIMP3
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.

      SUBUNIT STRUCTURE: Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. CARD11 and BCL10 bind to each other by CARD-CARD interaction. Interacts (via PDZ domain) with DPP4 (via cytoplasmic tail).

      SUBCELLULAR LOCATION: Cytoplasm. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.

      TISSUE SPECIFICITY: Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.

      SEQUENCE SIMILARITIES: Contains 1 CARD domain.

      Contains 1 guanylate kinase-like domain.

      Contains 1 PDZ (DHR) domain.

      CAUTION: Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.

      SEQUENCE CAUTION: The sequence AAQ96893.1 differs from that shown. Reason: Erroneous gene model prediction.

      The sequence EAL23962.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight~133 kDa.
      A non-specific band may be observed at ~51 kDa and >260kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Daudi cell lysate.

      Western Blot Analysis: 0.25 µg/mL of this antibody detected CARD11 in 10 µg of Daudi cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CARD11 - NRG1721909 NRG1721909

      References

      Reference overviewPub Med ID
      Gap junctional conductance between pairs of ventricular myocytes is modulated synergistically by H+ and Ca++.
      R L White,J E Doeller,V K Verselis,B A Wittenberg
      The Journal of general physiology  95  1990

      Show Abstract Full Text Article
      2115574 2115574