05-1410 | Anti-CD45 Antibody, clone F10-89-4

05-1410
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, WB, IF, IP, IH(P)MAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1410
      Replaces04-1102
      DescriptionAnti-CD45 Antibody, clone F10-89-4
      Alternate Names
      • CD45 antigen
      • Leukocyte common antigen precursor
      • SCID due to PTPRC deficiency
      • T200 glycoprotein
      • T200 leukocyte common antigen
      • Human homolog of severe combined immunodeficiency due to PTPRC deficiency
      • Leukocyte-common antigen
      • Protein tyrosine phosphatase, receptor type C
      • Protein tyrosine phosphatase, receptor type C polypeptide
      Background InformationCD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Jurkat cell lysate
      PresentationPurified mouse monoclonal IgG2a presented in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin.
      We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-CD45 Antibody, clone F10-89-4 detects level of CD45 & has been published & validated for use in FC, WB, IF, IP, IH(P).
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunofluorescence
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunofluorescence: A previous lot of this antibody was used in the identification of cells of leucocyte origin by indirect staining.

      Immunohistochemistry (paraffin): A previous lot of this antibody was used in the discrimination between malignant cells of haematopoietic origin and other malignancies in frozen tissue sections.

      Immunoprecipitation: A previous lot of this antibody was used in studies of the leucocyte common antigen complex and its identification by the Western blotting technique.

      Flow Cytometry: Studies of CD45 expression and the association with T-cell receptor signaling was performed using this antibody of previous lot.
      Studies of the mechanism by which CD45 regulates B cell activation through protein tyrosine phospatases.
      Optimal working dilutions must be determined by the end user.

      Immunohistochemistry(paraffin): Representative testing from a previous lot.

      Optimal Staining of CD45 Monoclonal Antibody: Tonsil
      Biological Information
      ImmunogenPurified T cells from human lymph nodes
      CloneF10-89-4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody reacts with the leucocyte common antigen (LCA) complex to be found on lymphocytes, monocytes, granulocytes, thymocytes and malignant T and B cells. No reactivity has been observed with primary or metastatic carcinoma cells. Plasma cells or myeloma cells may have weak expression or be negative for this antigen.
      The LCA comprises of at least 5 isoforms ranging from 180-220 kDa which are produced as a result of alternative splicing of exons A, B or C. CBL 124 recognizes epitopes common to
      all of the isoforms.
      Antigen distribution:
      Thymocytes > 95%
      Granulocytes > 95%
      Monocytes > 95%
      B cells (CD20+) > 95%
      T cells (CD3+) > 95%
      NK cells (CD16+) > 95%
      Peripheral blood lymphocytes > 95%
      FUSION PARTNER: NS1 myeloma cell line
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteMouse. Expected to react with human and rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
      Gene Symbol
      • PTPRC
      • L-CA
      • LCA
      • CD45
      • LY5
      • T200
      • Glycoprotein
      • B220
      • GP180
      • EC 3.1.3.48
      Purification MethodProtein A affinity chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08575 # Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
      SIZE: 1304 amino acids; 147254 Da
      SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1.
      PTM: Heavily N- and O-glycosylated.
      DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
      SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains.
      Molecular Weight~180-220 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on jurkat cell lysate.

      Western Blot Analysis: 1:500 dilution of this lot detected CD45 on 10 μg of Jurkat lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CD45, clone F10-89-4 - 2118993 2118993
      Anti-CD45, clone F10-89-4 - 2145735 2145735
      Anti-CD45, clone F10-89-4 - 2455624 2455624
      Anti-CD45, clone F10-89-4 - 2025437 2025437
      Anti-CD45, clone F10-89-4 - 2060506 2060506
      Anti-CD45, clone F10-89-4 - 2167186 2167186
      Anti-CD45, clone F10-89-4 - 2208786 2208786
      Anti-CD45, clone F10-89-4 - 2280476 2280476
      Anti-CD45, clone F10-89-4 - 2324658 2324658
      Anti-CD45, clone F10-89-4 - 2517821 2517821

      References

      Reference overviewApplicationPub Med ID
      Increased Infiltration of Extra-Cardiac Cells in Myxomatous Valve Disease.
      Sauls, K; Toomer, K; Williams, K; Johnson, AJ; Markwald, RR; Hajdu, Z; Norris, RA
      Journal of cardiovascular development and disease  2  200-213  2015

      Show Abstract
      26473162 26473162
      Regional heterogeneity in murine lung fibroblasts from normal mice or mice exposed once to cigarette smoke.
      Preobrazhenska, O; Wright, JL; Churg, A
      PloS one  7  e39761  2012

      Show Abstract
      Western Blotting22761892 22761892
      The effect of stromal cell-derived factor-1α/heparin coating of biodegradable vascular grafts on the recruitment of both endothelial and smooth muscle progenitor cells for accelerated regeneration.
      Jian Yu,Aijun Wang,Zhenyu Tang,Jeffrey Henry,Benjamin Li-Ping Lee,Yiqian Zhu,Failei Yuan,Fengping Huang,Song Li
      Biomaterials  33  2012

      Show Abstract
      22884813 22884813

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