05-1416 | Anti-CD45 Antibody, clone IBL-5/25

05-1416
500 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1416
      ReplacesCBL1326
      DescriptionAnti-CD45 Antibody, clone IBL-5/25
      Alternate Names
      • CD45 associated protein
      • CD45-associated protein
      • Lymphocyte phosphatase-associated phosphoprotein
      • PTPRC-associated protein
      • protein tyrosine phosphatase, receptor type, C-associated protein
      • protein tyrosine phosphatase, receptor type, c
        polypeptide-associated protein
      Background InformationCD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
      References
      Product Information
      FormatPurified
      Control
      • Mouse colon tissue
      PresentationPurified rat monoclonal IgG in phosphate buffered saline containing 10 mM sodium azide.
      Applications
      ApplicationAnti-CD45 Antibody, clone IBL-5/25 is an antibody against CD45 for use in FC, IH.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      Application NotesFlow Cytometry Analysis: A previous lot detected CD45, clone IBL-5/25 in mouse peripheral blood lymphocytes
      Biological Information
      ImmunogenMouse lymph node cells.
      CloneIBL-5/25
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityThis antibody reacts with all isoforms of mouse CD45 which is expressed on all haematopoietic cells except erythrocytes.
      FUSION PARTNER: SP2/0 myeloma cell line
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteReacts with mouse. Reactivity with other species has not been determined.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
      Gene Symbol
      • CD45-AP
      • LPAP
      • MGC138602
      • MGC138603
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummarySUBUNIT STRUCTURE: Interacts with CD45/PTPRC.
      SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein Potential.
      PTM: Phosphorylated on tyrosine residues.
      Molecular WeightApprox. 147 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse colon tissue.
      Immunohistochemistry Analysis: 1:100 dilution of this antibody detected CD45 in mouse colon tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C for 12 months from date of receipt.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CD45, clone IBL-5/25 2485683
      Anti-CD45, clone IBL-5/25 - 2395751 2395751
      Anti-CD45, clone IBL-5/25 - 1975096 1975096
      Anti-CD45, clone IBL-5/25 - 2042817 2042817
      Anti-CD45, clone IBL-5/25 - 2281697 2281697
      Anti-CD45, clone IBL-5/25 - JBC1780512 JBC1780512
      Anti-CD45, clone IBL-5/25 - NG1713105 NG1713105
      Anti-CD45, clone IBL-5/25 - NG1800888 NG1800888
      Anti-CD45, clone IBL-5/25 - NG1853790 NG1853790
      Anti-CD45, clone IBL-5/25 - NG1894404 NG1894404

      References

      Reference overviewPub Med ID
      Merkel Cell Polyomavirus Small T Antigen Induces Cancer and Embryonic Merkel Cell Proliferation in a Transgenic Mouse Model.
      Shuda, M; Guastafierro, A; Geng, X; Shuda, Y; Ostrowski, SM; Lukianov, S; Jenkins, FJ; Honda, K; Maricich, SM; Moore, PS; Chang, Y
      PloS one  10  e0142329  2015

      Show Abstract
      26544690 26544690
      Impaired autophagy contributes to adverse cardiac remodeling in acute myocardial infarction.
      Wu, X; He, L; Chen, F; He, X; Cai, Y; Zhang, G; Yi, Q; He, M; Luo, J
      PloS one  9  e112891  2014

      Show Abstract
      25409294 25409294
      Oxidative stress, inflammation, and autophagic stress as the key mechanisms of premature age-related hearing loss in SAMP8 mouse Cochlea.
      Menardo, Julien, et al.
      Antioxid. Redox Signal., 16: 263-74 (2012)  2012

      Show Abstract
      21923553 21923553