CBL458 | Anti-CD49f Antibody, clone 4F10

CBL458
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IP, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL458
      ReplacesMAB2254
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CD49f Antibody, clone 4F10
      Alternate Names
      • Integrin alpha6
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal was purified by protein A affinity chromatography and is presented as a liquid in phosphate buffered saline containing 10mM sodium azide and 1 mg/mL bovine serum albumin.
      Applications
      ApplicationThis Anti-CD49f Antibody, clone 4F10 is validated for use in FC, IP, IH for the detection of CD49f.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesImmunohistological studies on frozen sections

      Immunoprecipitation of the laminin receptor

      Flow Cytometry

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenCell line SW/222 (Human colorectal carcinoma)
      Clone4F10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody is specific to the integrin alpha6 chain which is expressed on epithelial and endothelial basement membrane. The VLA alpha6 chain, which consists of a single disulphide linked 120 kDa subunit and a small doublet subunit of 25/30 kDa, is non covalently associated with the VLA beta-chain (CD29) in VLA-6 or alpha6/beta1 complex or with integrin beta4 chain (alpha6/beta4).

      The antigen is also expressed on platelets, monocytes, thymocytes and T lymphocytes.

      FUSION PARTNER: NS1 myeloma cell line
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • ITGA6
      • VLA-6
      • ITGA6B
      • CD49f
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23229 # Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome.
      SIZE: 1130 amino acids; 126619 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
      PTM: Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
      DISEASE: "SwissProt: P23229 # Defects in ITGA6 are a cause of epidermolysis bullosa with pyloric atresia (EB-PA) [MIM:226730]; also known as aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive disease characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus."
      SIMILARITY: SwissProt: P23229 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore an 2-8°C for up to 12 months from date of receipt. For long term storage aliquot antibody into small volumes and store at -20°C. Avoid repeated freeze-thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN CD49f -2499675 2499675
      MOUSE ANTI-HUMAN CD49f -2583993 2583993
      MOUSE ANTI-HUMAN CD49f -2585783 2585783
      MOUSE ANTI-HUMAN CD49f -2638885 2638885
      MOUSE ANTI-HUMAN CD49f -2739307 2739307
      MOUSE ANTI-HUMAN CD49f -2787706 2787706
      MOUSE ANTI-HUMAN CDW49f MONOCLONAL ANTIBODY - 2119731 2119731
      MOUSE ANTI-HUMAN CDW49f MONOCLONAL ANTIBODY - 2377182 2377182
      MOUSE ANTI-HUMAN CDW49f MONOCLONAL ANTIBODY - 1987048 1987048
      MOUSE ANTI-HUMAN CDW49f MONOCLONAL ANTIBODY - 2204909 2204909

      References

      Reference overviewPub Med ID
      Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.
      Zhao, X; Malhotra, GK; Band, H; Band, V
      PloS one  7  e35338  2012

      Show Abstract
      22514728 22514728
      Telomerase-immortalized human mammary stem/progenitor cells with ability to self-renew and differentiate.
      Zhao X, Malhotra GK, Lele SM, Lele MS, West WW, Eudy JD, Band H, Band V
      Proc Natl Acad Sci U S A  107  14146-51. Epub 2010 Jul 26.  2010

      Show Abstract
      20660721 20660721
      Mannose 6-phosphate/insulin-like growth factor 2 receptor limits cell invasion by controlling alphaVbeta3 integrin expression and proteolytic processing of urokinase-type plasminogen activator receptor.
      Schiller, HB; Szekeres, A; Binder, BR; Stockinger, H; Leksa, V
      Molecular biology of the cell  20  745-56  2009

      Show Abstract Full Text Article
      19037107 19037107
      Laminin-5 induces osteogenic gene expression in human mesenchymal stem cells through an ERK-dependent pathway.
      Klees, RF; Salasznyk, RM; Kingsley, K; Williams, WA; Boskey, A; Plopper, GE
      Molecular biology of the cell  16  881-90  2005

      Show Abstract
      15574877 15574877
      Expression of integrin subunits in the human infant breast correlates with morphogenesis and differentiation
      Anbazhagan, R. et al.
      J. Path., 176 (3):227-232 (1995)  1995

      7674085 7674085
      Cell adhesion molecules in oesophageal epithelium
      Dobson, H. et al.
      GUT, 35:1343-1347 (1994)  1994

      7959182 7959182
      Integrins and their accessory adhesion molecules in mammary carcinomas: loss of polarization in poorly differentiated tumors.
      Pignatelli, M, et al.
      Hum. Pathol., 23: 1159-66 (1992)  1992

      Show Abstract
      1383121 1383121

      Data Sheet

      Title
      MOUSE ANTI-HUMAN CDW49f

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      Alternative Format

      Catalogue Number Description  
      CBL458F Anti-CD49f Antibody, FITC conjugated, clone 4F10, FITC conjugated Show Pricing & Availability
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies