06-1398 | Anti-CDK5RAP2 Antibody

06-1398
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1398
      DescriptionAnti-CDK5RAP2 Antibody
      Alternate Names
      • CDK5 regulatory subunit-associated protein 2
      • CDK5 activator-binding protein C48
      • Centrosome-associated protein 215
      Background InformationAlso known as Cep215, CDK5RAP2 is involved in a variety of cellular processes. It serves as a potential regulator of CDK5 activity through its interaction with CDK5R1. It acts as a negative regulator of centriole disengagement, which maintains centriole engagement and cohesion. It is involved in the regulation of mitotic spindle orientation. Additionally, this protein mediates spindle checkpoint activation by serving as a transcriptional regulator of the MAD2 and BUBR1 promoter. In conjuction with MAPRE1, CDK5RAP2 promotes microtubule polymerization, bundle formation, and growth at the plus ends. It also plays a role in DNA damage-induced G2 cell cycle checkpoint. CDK5RAP2 is known to be mutated in primary microcephaly, a neuodevelopmental disorder that is characterized by a decrease in brain size.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-CDK5RAP2 Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect CDK5RAP2 also known as CDK5 regulatory subunit-associated protein 2, CDK5 activator-binding protein C48, Centrosome-associated protein 215.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot from an independent laboratory detected CDK5RAP2 in RPE1 cells. (Barr, A.R., et al. (2010). J Cell Biol. 189(1):23-39.)
      Biological Information
      ImmunogenMBP-tagged recombinant protein corresponding to human CDK5RAP2.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Other Homologies: Rat (83% sequence homology) and Mouse (82% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNeuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. The encoded protein may also be a substrate of neuronal CDC2-like kinase. Multiple transcript variants exist for this gene, but the full-length nature of only two has been determined.
      Gene Symbol
      • CDK5RAP2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation. Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends.

      SUBUNIT STRUCTURE: Interacts with CDK5R1 (p35 form). CDK5RAP1, CDK5RAP2 and CDK5RAP3 show competitive binding to CDK5R1. Probably forms a complex with CDK5R1 and CDK5. Interacts with PCNT; the interaction is leading to centrosomal and Golgi localization of CDK5RAP2 and PCNT. Interacts with AKAP9; the interaction is leading to Golgi localization of CDK5RAP2 and AKAP9. Interacts with MAPRE1; the interaction is leading to microtubule attachment at plus ends of CDK5RAP2 and MAPRE1. Interacts with TUBG1; the interaction is leading to the centrosomal localization of CDK5RAP2 and TUBG1. Interacts with TUBGCP3. Interacts with CALM1. Interacts with CDC20.

      SUBCELLULAR LOCATION: Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasm. Note: Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends.

      TISSUE SPECIFICITY: Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
      Molecular Weight~220 kDa observed. Uniprot.org states four isoforms produced by alternative splicing, which may be observed at a molecular weight of ~216 kDa, ~211 kDa, ~210 kDa, and ~206 kDa in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in 10 µg of HeLa cell lysate.

      Western Blot Analysis: A 1:2,500 dilution of this antibody dectected CDK5RAP2 in 10µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CDK5RAP2 - 2211074 2211074
      Anti-CDK5RAP2 - 2298396 2298396
      Anti-CDK5RAP2 - NRG1950705 NRG1950705
      Anti-CDK5RAP2 -2520501 2520501
      Anti-CDK5RAP2 -2605481 2605481
      Anti-CDK5RAP2 -2637520 2637520
      Anti-CDK5RAP2 -2660184 2660184
      Anti-CDK5RAP2 -2695271 2695271
      Anti-CDK5RAP2 -2794942 2794942
      Anti-CDK5RAP2 Polyclonal Antibody 3002808

      References

      Reference overviewPub Med ID
      Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation.
      Shukla, A; Kong, D; Sharma, M; Magidson, V; Loncarek, J
      Nature communications  6  8077  2015

      Show Abstract
      26293378 26293378
      CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly.
      Ohta, S; Wood, L; Toramoto, I; Yagyu, K; Fukagawa, T; Earnshaw, WC
      Molecular biology of the cell  26  1225-37  2015

      Show Abstract
      25657325 25657325
      Loss of centrioles causes chromosomal instability in vertebrate somatic cells.
      Sir, Joo-Hee, et al.
      J. Cell Biol., (2013)  2013

      Show Abstract
      24297747 24297747
      CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response.
      Barr, Alexis R, et al.
      J. Cell Biol., 189: 23-39 (2010)  2010

      Show Abstract
      20368616 20368616

      Brochure

      Title
      New Products: Volume 3, 2012