07-344 | Anti-CPI-17 Antibody

07-344
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Po, R, RbWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-344
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CPI-17 Antibody
      References
      Product Information
      FormatPurified
      PresentationPBS, 0.02% sodium azide before the addition of glycerol to 10%
      Applications
      ApplicationAnti-CPI-17 Antibody is an antibody against CPI-17 for use in WB.
      Key Applications
      • Western Blotting
      Application NotesNOT cross-reactive in yeast.
      Biological Information
      ImmunogenRecombinant protein, full length pig CPI-17
      HostRabbit
      SpecificityCPI-17
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM]
      Gene Symbol
      • PPP1R14A
      • CPI-17
      • PPP1INL
      • 17-KDasprotein
      • CPI17
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q96A00 # Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
      SIZE: 147 amino acids; 16693 Da
      SUBCELLULAR LOCATION: Cytoplasm (By similarity).
      TISSUE SPECIFICITY: Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.
      SIMILARITY: SwissProt: Q96A00 ## Belongs to the PP1 inhibitor family.
      Molecular Weight22kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from mouse brain nuclear fraction and pig carotid artery tissue extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CPI-17 (rabbit polyclonal IgG) - 2137088 2137088
      Anti-CPI-17 (rabbit polyclonal IgG) - 2395753 2395753
      Anti-CPI-17 (rabbit polyclonal IgG) 3116320
      Anti-CPI-17 (rabbit polyclonal IgG) 2991968
      Anti-CPI-17 (rabbit polyclonal IgG) - 1966950 1966950
      Anti-CPI-17 (rabbit polyclonal IgG) - JBC1381981 JBC1381981
      Anti-CPI-17 - 2043527 2043527
      Anti-CPI-17 - 22343 22343
      Anti-CPI-17 - 2510378 2510378
      Anti-CPI-17 - 26462 26462

      References

      Reference overviewApplicationSpeciesPub Med ID
      The role of calcium-independent phospholipase A2γ in modulation of aqueous humor drainage and Ca2+ sensitization of trabecular meshwork contraction.
      Pattabiraman, PP; Lih, FB; Tomer, KB; Rao, PV
      American journal of physiology. Cell physiology  302  C979-91  2012

      Show Abstract
      22237407 22237407
      Ca2+-independent, inhibitory effects of cyclic adenosine 5'-monophosphate on Ca2+ regulation of phosphoinositide 3-kinase C2alpha, Rho, and myosin phosphatase in vascular smooth muscle.
      Azam, MA; Yoshioka, K; Ohkura, S; Takuwa, N; Sugimoto, N; Sato, K; Takuwa, Y
      The Journal of pharmacology and experimental therapeutics  320  907-16  2007

      Show Abstract
      Western BlottingMouse17110524 17110524
      Class II phosphoinositide 3-kinase alpha-isoform regulates Rho, myosin phosphatase and contraction in vascular smooth muscle.
      Yu Wang,Kazuaki Yoshioka,Mohammed Ali Azam,Noriko Takuwa,Sotaro Sakurada,Yuji Kayaba,Naotoshi Sugimoto,Isao Inoki,Takaharu Kimura,Tomoyuki Kuwaki,Yoh Takuwa
      The Biochemical journal  394  2006

      Show Abstract Full Text Article
      16336212 16336212
      Disruption of Rho signal transduction upon cell detachment.
      Ren, XD; Wang, R; Li, Q; Kahek, LA; Kaibuchi, K; Clark, RA
      Journal of cell science  117  3511-8  2004

      Show Abstract
      15226371 15226371
      Cerebellar long-term synaptic depression requires PKC-mediated activation of CPI-17, a myosin/moesin phosphatase inhibitor
      Eto, M., et al
      Neuron, 36:1145-58 (2002)  2002

      Immunoprecipitation12495628 12495628
      Histamine-induced vasoconstriction involves phosphorylation of a specific inhibitor protein for myosin phosphatase by protein kinase C alpha and delta isoforms.
      Eto, M, et al.
      J. Biol. Chem., 276: 29072-8 (2001)  2001

      Show Abstract
      Kinase Assay11397799 11397799
      Solution NMR structure of the myosin phosphatase inhibitor protein CPI-17 shows phosphorylation-induced conformational changes responsible for activation.
      Ohki, S, et al.
      J. Mol. Biol., 314: 839-49 (2001)  2001

      Show Abstract
      11734001 11734001
      Molecular cloning of a novel phosphorylation-dependent inhibitory protein of protein phosphatase-1 (CPI17) in smooth muscle: its specific localization in smooth muscle.
      Eto, M, et al.
      FEBS Lett., 410: 356-60 (1997)  1997

      9237662 9237662

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies